Abstract
Pulse Field Gel Electrophoresis (PFGE), unlike conventional electrophoresis, can resolve DNA fragments greater than 30 kb and is a highly discriminatory molecular typing method. Here we describe a PFGE protocol for bifidobacteria characterized by a short lysis time determined by the addition of lysis reagents to the initial cell suspension, a reduced incubation period of the plugs in proteinase K, and an improved washing plug step with preheating of the buffer in a shaking incubator.
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Jiménez, E., Gómez, M., Moles, L. (2015). Pulsed Field Gel Electrophoresis for Bifidobacterium . In: Jordan, K., Dalmasso, M. (eds) Pulse Field Gel Electrophoresis. Methods in Molecular Biology, vol 1301. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2599-5_20
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DOI: https://doi.org/10.1007/978-1-4939-2599-5_20
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2598-8
Online ISBN: 978-1-4939-2599-5
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