Abstract
Metabonomics aims at a comprehensive and semiquantitative monitoring of the perturbations of the metabonome in response to pathophysiological stimuli. Liquid chromatography-mass spectrometry (LC-MS) is one of the most commonly employed analytical platforms for this purpose. As the sample pretreatment is detailed in the former chapter, we here describe the practical procedures for the LC-MS-based metabolic profiling of biological samples including detailed liquid chromatographic and MS conditions, batch analysis, peak alignment, data quality assessment, and compound identification.
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Acknowledgements
This study was supported by the National Basic Research Program (2012CB518303 and No. 2012CB517506) from the State Ministry of Science and Technology of China, the grants (Nos. 21275141) and 21435006) from the National Natural Science Foundation of China.
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Zhao, X., Zhou, L., Yin, P., Xu, G. (2015). Liquid Chromatography-Mass Spectrometry of Biofluids and Extracts. In: Bjerrum, J. (eds) Metabonomics. Methods in Molecular Biology, vol 1277. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2377-9_6
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DOI: https://doi.org/10.1007/978-1-4939-2377-9_6
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