Abstract
Microvascularity and angiogenesis play a pivotal role during normal growth and in a variety of pathological conditions such as inflammation, tumor growth, macular degeneration, and tissue regeneration. Vascular corrosion casting has been established as a method to analyze and evaluate two- and three-dimensionally the morphology and architecture of blood vessels of organs and tissues, such as tumors, brains, embryos, or the chorioallantoic membrane. Microvascular casts may be further dissected for visualizing and quantifying vascular morphology using scanning electron microscopy (SEM), micro computed tomographic (μCT) imaging, or synchrotron radiation-based micro computed tomographic (SRμCT) imaging.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Ausprunk DH, Folkman J (1977) Migration and proliferation of endothelial cells in preformed and newly formed blood vessels during tumor angiogenesis. Microvasc Res 14: 53–65
Risau W (1997) Mechanisms of angiogenesis. Nature 386:671–674
Patan S (2000) Vasculogenesis and angiogenesis as mechanisms of vascular network formation, growth and remodeling. J Neurooncol 50:1–15
Murakami T (1971) Application of the scanning electron microscope to the study of the fine distribution of the blood vessels. Arch Histol Jpn 32:445–454
Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671–675
De Smet F, Segura I, De Bock K, Hohensinner PJ, Carmeliet P (2009) Mechanisms of vessel branching: filopodia on endothelial tip cells lead the way. Arterioscler Thromb Vasc Biol 29:639–649
Ackermann M, Houdek JP, Gibney BC, Ysasi A, Wagner W, Belle J et al (2014) Sprouting and intussusceptive angiogenesis in postpneumonectomy lung growth: mechanisms of alveolar neovascularization. Angiogenesis 17(3): 541–551
Styp-Rekowska B, Hlushchuk R, Pries AR, Djonov V (2011) Intussusceptive angiogenesis: pillars against the blood flow. Acta Physiol (Oxf) 202:213–223
Hlushchuk R, Riesterer O, Baum O, Wood J, Gruber G, Pruschy M et al (2008) Tumor recovery by angiogenic switch from sprouting to intussusceptive angiogenesis after treatment with PTK787/ZK222584 or ionizing radiation. Am J Pathol 173:1173–1185
Dimova I, Hlushchuk R, Makanya A, Styp-Rekowska B, Ceausu A, Flueckiger S et al (2013) Inhibition of Notch signaling induces extensive intussusceptive neo-angiogenesis by recruitment of mononuclear cells. Angiogenesis 16:921–937
Konerding MA, Turhan A, Ravnic DJ, Lin M, Fuchs C, Secomb TW, Tsuda A, Mentzer SJ (2010) Inflammation-induced intussusceptive angiogenesis in murine colitis. Anat Rec (Hoboken) 293:849–857
Lee GS, Filipovic N, Miele LF, Lin M, Simpson DC, Giney B et al (2010) Blood flow shapes intravascular pillar geometry in the chick chorioallantoic membrane. J Angiogenes Res 2:11
Filipovic N, Tsuda A, Lee GS, Miele LF, Lin M, Konerding MA et al (2009) Computational flow dynamics in a geometric model of intussusceptive angiogenesis. Microvasc Res 78: 286–293
Frid MG, Brunetti JA, Burke DL, Carpenter TC, Davie NJ, Reeves JT et al (2006) Hypoxia-induced pulmonary vascular remodeling requires recruitment of circulating mesenchymal precursors of a monocyte/macrophage lineage. Am J Pathol 168:659–669
Secomb TW, Konerding MA, West CA, Su M, Young AJ, Mentzer SJ (2003) Microangiectasias: structural regulators of lymphocyte transmigration. Proc Natl Acad Sci U S A 100: 7231–7234
Erba P, Ogawa R, Ackermann M, Adini A, Miele LF, Dastouri P et al (2011) Angiogenesis in wounds treated by microdeformational wound therapy. Ann Surg 253:402–409
Vagnucci AH Jr, Li WW (2003) Alzheimer’s disease and angiogenesis. Lancet 361:605–608
Miyakawa T (2010) Vascular pathology in Alzheimer’s disease. Psychogeriatrics 10:39–44
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Ackermann, M., Konerding, M.A. (2015). Vascular Casting for the Study of Vascular Morphogenesis. In: Ribatti, D. (eds) Vascular Morphogenesis. Methods in Molecular Biology, vol 1214. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1462-3_5
Download citation
DOI: https://doi.org/10.1007/978-1-4939-1462-3_5
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1461-6
Online ISBN: 978-1-4939-1462-3
eBook Packages: Springer Protocols