Abstract
Microfluidic systems have emerged as an important technology for modeling cellular microenvironments in vitro. These systems enable unprecedented levels of control of chemical gradients, fluid flow, and localized 3-D extracellular matrices (ECM), all of which can be integrated to provide a physiologically relevant context for studying complex cellular processes such as angiogenesis. Here, we describe the design and use of microfluidic systems for reproducing the dynamic events of vascular morphogenesis.
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References
Inamdar NK, Borenstein JT (2011) Microfluidic cell culture models for tissue engineering. Curr Opin Biotechnol 22:681–689
Stone HA, Stroock AD, Ajdari A (2004) Engineering flows in small devices: microfluidics toward a lab-on-a-chip. Annu Rev Fluid Mech 36:381–411
Whitesides GM (2006) The origins and the future of microfluidics. Nature 442:368–373
Duffy DC, McDonald JC, Schueller OJA, Whitesides GM (1998) Rapid prototyping of microfluidic systems in poly(dimethylsiloxane). Anal Chem 70:4974–4984
Whitesides GM, Ostuni E, Takayama S, Jiang X, Ingber DE (2001) Soft lithography in biology and biochemistry. Annu Rev Biomed Eng 3:335–373
El-Ali J, Sorger PK, Jensen KF (2006) Cells on chips. Nature 442:403–411
Young EW, Simmons CA (2010) Macro- and microscale fluid flow systems for endothelial cell biology. Lab Chip 10:143–160
Kim L, Toh YC, Voldman J, Yu H (2007) A practical guide to microfluidic perfusion culture of adherent mammalian cells. Lab Chip 7:681–694
Walker GM, Zeringue HC, Beebe DJ (2004) Microenvironment design considerations for cellular scale studies. Lab Chip 4:91–97
Mosadegh B, Huang C, Park JW, Shin HS, Chung BG, Hwang SK et al (2007) Generation of stable complex gradients across two-dimensional surfaces and three-dimensional gels. Langmuir 23:10910–10912
Shamloo A, Heilshorn SC (2010) Matrix density mediates polarization and lumen formation of endothelial sprouts in VEGF gradients. Lab Chip 10:3061–3068
Young EW, Wheeler AR, Simmons CA (2007) Matrix-dependent adhesion of vascular and valvular endothelial cells in microfluidic channels. Lab Chip 7:1759–1766
Song JW, Gu W, Futai N, Warner KA, Nor JE, Takayama S (2005) Computer-controlled microcirculatory support system for endothelial cell culture and shearing. Anal Chem 77:3993–3999
Andersson H, Berg A (2004) Microfabrication and microfluidics for tissue engineering: state of the art and future opportunities. Lab Chip 4:98–103
Song JW, Bazou D, Munn LL (2012) Anastomosis of endothelial sprouts forms new vessels in a tissue analogue of angiogenesis. Integr Biol (Camb) 4:857–862
Song JW, Munn LL (2011) Fluid forces control endothelial sprouting. Proc Natl Acad Sci U S A 108:15342–15347
Song JW, Daubriac J, Tse JM, Bazou D, Munn LL (2012) RhoA mediates flow-induced endothelial sprouting in a 3-D tissue analogue of angiogenesis. Lab Chip 12:5000–5006
Qin D, Xia Y, Whitesides GM (2010) Soft lithography for micro- and nanoscale patterning. Nat Protocols 5:491–502
Morgan JP, Delnero PF, Zheng Y, Verbridge SS, Chen J, Craven M et al (2013) Formation of microvascular networks in vitro. Nat Protocols 8:1820–1836
Walker GM, Beebe DJ (2002) A passive pumping method for microfluidic devices. Lab Chip 2:131–134
Vestweber D (2008) VE-cadherin: the major endothelial adhesion molecule controlling cellular junctions and blood vessel formation. Arterioscler Thromb Vasc Biol 28:223–232
Huh D, Mills K, Zhu X, Burns MA, Thouless M, Takayama S (2007) Tuneable elastomeric nanochannels for nanofluidic manipulation. Nat Mater 6:424–428
Chaw KC, Manimaran M, Tay FE, Swaminathan S (2007) Matrigel coated polydimethylsiloxane based microfluidic devices for studying metastatic and non-metastatic cancer cell invasion and migration. Biomed Microdevices 9:597–602
Huang CP, Lu J, Seon H, Lee AP, Flanagan LA, Kim HY et al (2009) Engineering microscale cellular niches for three-dimensional multicellular co-cultures. Lab Chip 9:1740–1748
Vickerman V, Blundo J, Chung S, Kamm R (2008) Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging. Lab Chip 8:1468–1477
Atherton A, Born GV (1973) Relationship between the velocity of rolling granulocytes and that of the blood flow in venules. J Physiol 233:157–165
Munn LL (2003) Aberrant vascular architecture in tumors and its importance in drug-based therapies. Drug Discov Today 8:396–403
Acknowledgement
We acknowledge support from grants from the National Institutes of Health: R01CA149285 (LLM) and T32CA073479.
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Song, J.W., Bazou, D., Munn, L.L. (2015). Microfluidic Model of Angiogenic Sprouting. In: Ribatti, D. (eds) Vascular Morphogenesis. Methods in Molecular Biology, vol 1214. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1462-3_15
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DOI: https://doi.org/10.1007/978-1-4939-1462-3_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-1462-3
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