Abstract
The Microarray Assay for Realtime Coregulator-Nuclear receptor Interaction (MARCoNI) technology allows the identification of nuclear receptor–coregulator interactions via flow-through microarrays. As such, differences in the coregulator profile between distinct nuclear receptors or of a single receptor in agonist or antagonist mode can be investigated, even in a single run. In this chapter, the method how to perform these peptide microarrays with cell lysates containing the overexpressed glucocorticoid receptor is described, as well as the influence of assay parameters, variations to the protocol, and data analysis.
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© 2014 Springer Science+Business Media New York
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Desmet, S.J. et al. (2014). Cofactor Profiling of the Glucocorticoid Receptor from a Cellular Environment. In: Castoria, G., Auricchio, F. (eds) Steroid Receptors. Methods in Molecular Biology, vol 1204. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-1346-6_8
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DOI: https://doi.org/10.1007/978-1-4939-1346-6_8
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-1345-9
Online ISBN: 978-1-4939-1346-6
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