Abstract
The use of animal models is a key step to better understand bacterial virulence factors and their roles in host/pathogen interactions. To avoid the ethical and cost problems of mammalian models in bacterial virulence research, several insect models have been developed. One of these models, the larvae of the greater wax moth Galleria mellonella, has been shown to be relevant for several fungal and bacterial mammalian pathogens. Here, we describe the use G. mellonella to study virulence of the highly virulent facultative intracellular bacterial pathogens: Brucella suis, Brucella melitensis, Francisella tularensis, Burkholderia mallei, and Burkholderia pseudomallei.
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Abbreviations
- TS:
-
Trypticase soy
- BHI:
-
Brain–heart infusion
- CFU:
-
Colony-forming units
- MOI:
-
Multiplicity of infection
- PBS:
-
Phosphate-buffered saline
References
Scully LR, Bidochka MJ (2006) Developing insect models for the study of current and emerging human pathogens. FEMS Microbiol Lett 263(1):1–9
Dunphy GB, Oberholzer U, Whiteway M, Zakarian RJ, Boomer I (2003) Virulence of Candida albicans mutants toward larval Galleria mellonella (Insecta, Lepidoptera, Galleridae). Can J Microbiol 49(8):514–524
Mylonakis E et al (2005) Galleria mellonella as a model system to study Cryptococcus neoformans pathogenesis. Infect Immun 73(7):3842–3850
Reeves EP, Messina CG, Doyle S, Kavanagh K (2004) Correlation between gliotoxin production and virulence of Aspergillus fumigatus in Galleria mellonella. Mycopathologia 158(1):73–79
Champion OL et al (2009) Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis. Microbiology 155(Pt 5):1516–1522
Harding CR et al (2012) Legionella pneumophila pathogenesis in the Galleria mellonella infection model. Infect Immun 80(8):2780–2790
Mukherjee K et al (2010) Galleria mellonella as a model system for studying Listeria pathogenesis. Appl Environ Microbiol 76(1):310–317
Seed KD, Dennis JJ (2008) Development of Galleria mellonella as an alternative infection model for the Burkholderia cepacia complex. Infect Immun 76(3):1267–1275
Kavanagh K, Reeves EP (2004) Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens. FEMS Microbiol Rev 28(1):101–112
Jander G, Rahme LG, Ausubel FM (2000) Positive correlation between virulence of Pseudomonas aeruginosa mutants in mice and insects. J Bacteriol 182(13):3843–3845
Ramarao N, Nielsen-Leroux C, Lereclus D (2012) The insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis. J Vis Exp 70:e4392
Banville N, Browne N, Kavanagh K (2012) Effect of nutrient deprivation on the susceptibility of Galleria mellonella larvae to infection. Virulence 3(6):497–503
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© 2014 Springer Science+Business Media New York
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Sprynski, N., Valade, E., Neulat-Ripoll, F. (2014). Galleria mellonella as an Infection Model for Select Agents. In: Vergunst, A., O'Callaghan, D. (eds) Host-Bacteria Interactions. Methods in Molecular Biology, vol 1197. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1261-2_1
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DOI: https://doi.org/10.1007/978-1-4939-1261-2_1
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1260-5
Online ISBN: 978-1-4939-1261-2
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