Abstract
Changes in metabolite concentrations in response to specific diseases, treatments, diets, or other factors can be used to understand the complex mechanisms that control and regulate the human body and potentially detect the onset of disease prior to the observation of symptoms in a patient. Different analytical and chemometric platforms are necessary to detect as many metabolites as possible in different biological fluids.
Capillary electrophoresis (CE) coupled to mass spectrometry (MS) is a particularly attractive, although still not common, approach for metabolomics for the detection of mainly polar and ionic metabolites. Among its main features, CE provides the capability to separate complex mixtures with high resolution and minimum sample treatment. However, the routine, automated use of CE-MS is not without challenges. In this chapter we describe a well-tested method for fingerprinting serum and urine using CE-TOF-MS. We describe below a sensitive and quite robust method for metabolomics with CE-MS including sample treatment, separation conditions, instrumental setup, and identification of 76 metabolites in the profile. Useful advice for daily practice is also included for every step of the procedure.
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Acknowledgements
Shama Naz receiving funding from the European Union Seventh Framework Programme [FP7/2007-2013] under grant agreement no. 264864. The authors gratefully also acknowledge the financial support from Ministerio de Economía y Competitividad (previously Ciencia y Tecnología) grant MCIT CTQ2011-23562.
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Garcia, A., Naz, S., Barbas, C. (2014). Metabolite Fingerprinting by Capillary Electrophoresis-Mass Spectrometry. In: Raftery, D. (eds) Mass Spectrometry in Metabolomics. Methods in Molecular Biology, vol 1198. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1258-2_8
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DOI: https://doi.org/10.1007/978-1-4939-1258-2_8
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