Abstract
Bacteriophages able to propagate on Pseudomonas strains are very common and can be easily isolated from natural environments or lysogenic strains. The development of transducing systems has allowed bacterial geneticists to perform chromosome analyses and mutation mapping. Moreover, these systems have also been proved to be a successful tool for molecular microbiologists to introduce a foreign gene or a mutation into the chromosome of a bacterial cell. This chapter provides a description of the phage methodology illustrated by Adams in 1959 and applicable to strain PAO1 derivatives.
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References
Zinder ND, Lederberg J (1952) Genetic exchange in Salmonella. J Bacteriol 64:679–699
Adams MH (1959) Bacteriophages. Interscience, New York
Morse ML, Lederberg EM, Lederberg J (1956) Transduction in Escherichia coli K-12. Genetics 41:142–156
Lederberg EM, Lederberg J (1953) Genetic studies of lysogenicity in Escherichia coli. Genetics 38:51–64
Holloway BW (1969) Genetics of Pseudomonas. Bacteriol Rev 33:419–443
Holloway BW, Krishnapillai V, Morgan AF (1979) Chromosomal genetics of Pseudomonas. Microbiol Rev 43:73–102
Krishnapillai V (1971) A novel transducing phage. Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa. Mol Gen Genet 114:134–143
Holloway BW, van de Putte P (1968) Lysogeny and bacterial recombination. In: Peacock WJ, Brock RD (eds) Replication and recombination of genetic material. Australian Academy of Sciences, Canberra, pp 175–183
Holloway BW, Krishnapillai V (1975) Bacteriophages and bacteriocins. In: Clarke PH, Richmond MH (eds) Genetics and biochemistry of Pseudomonas. John Wiley and Sons, London, pp 99–132
Fargie B, Holloway BW (1965) Absence of clustering of functionally related genes in Pseudomonas aeruginosa. Genet Res 6:284–299
Fox A, Haas D, Reimmann C et al (2008) Emergence of secretion-defective sublines of Pseudomonas aeruginosa PAO1 resulting from spontaneous mutations in the vfr global regulatory gene. Appl Environ Microbiol 74:1902–1908
Haas D, Holloway BW, Schomböch A et al (1977) The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa. Mol Gen Genet 154:7–22
Mee BJ, Lee BTO (1967) An analysis of histidine requiring mutants in Pseudomonas aeruginosa. Genetics 55:709–722
Morgan AF (1979) Transduction of Pseudomonas aeruginosa with a mutant of bacteriophage E79. J Bacteriol 139:137–140
Malone JG, Jaeger T, Spangler C et al (2010) YfiBNR mediates cyclic di-GMP dependent small colony variant formation and persistence in Pseudomonas aeruginosa. PLoS Pathog 12(6(3)):1000804
Valentini M, Storelli N, Lapouge K (2011) Identification of C(4)-dicarboxylate transport systems in Pseudomonas aeruginosa PAO1. J Bacteriol 193:4307–4316
Pemberton JM (1973) F116: a DNA bacteriophage specific for the pili of Pseudomonas aeruginosa strain PAO. Virology 55:558–560
Jarrell K, Kropinski AM (1977) Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO. J Virol 23:461–466
Stanisich VA, Holloway BW (1972) A mutant sex factor of Pseudomonas aeruginosa. Genet Res 19:91–108
Acknowledgements
I wish to thank Dr Áine Fox, with whom I had the pleasure of working on several transduction experiments whilst at the University of Lausanne, for critically reviewing this book chapter and Professor Dieter Haas, my Ph.D. supervisor, for introducing me to the fascinating world of phages.
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Frangipani, E. (2014). Gene Transfer: Transduction. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_1
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_1
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