Skip to main content

TILLING for Plant Breeding

  • Protocol
  • First Online:
Crop Breeding

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1145))

Abstract

TILLING is widely used in plant functional genomics. Mutagenesis and SNP detection is combined to allow for the isolation of mutations in genes of interest. It can also be used as a plant breeding tool, whereby variation in known or candidate genes of interest to breeding programs is generated. Here we describe a simple low-cost TILLING procedure.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. McCallum CM, Comai L, Greene EA, Henikoff S (2000) Targeted screening for induced mutations. Nat Biotechnol 18:455–457

    Article  CAS  PubMed  Google Scholar 

  2. McCallum CM, Comai L, Greene EA, Henikoff S (2000) Targeting induced local lesions in genomes (TILLING) for plant functional genomics. Plant Physiol 123:439–442

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  3. Colbert T, Till BJ, Tompa R, Reynolds S, Steine MN, Yeung AT, McCallum CM, Comai L, Henikoff S (2001) High-throughput screening for induced point mutations. Plant Physiol 126:480–484

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Wang T, Uauy C, Till B, Liu C-M (2010) TILLING and associated technologies. J Integr Plant Biol 52:2017–2030

    Google Scholar 

  5. Uauy C, Paraiso F, Colasuonno P, Tran RK, Tsai H, Berardi S, Comai L, Dubcovsky J (2009) A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat. BMC Plant Biol. doi:10.1186/1471-2229-9-115

    PubMed Central  PubMed  Google Scholar 

  6. Dong C, Vincent K, Sharp P (2009) Simultaneous mutation detection of three homoeologous genes in wheat by High Resolution Melting analysis and Mutation Surveyor®. BMC Plant Biol. doi:10.1186/1471-2229-9-143

    PubMed Central  PubMed  Google Scholar 

  7. Dong C, Dalton-Morgan C, Vincent K, Sharp P (2009) A modified TILLING method for wheat breeding. Plant Genome 2:39–47

    Article  CAS  Google Scholar 

  8. Slade AJ, Fuerstenberg SI, Loeffler D, Steine MN, Facciotti D (2005) A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nat Biotechnol 23:75–81

    Article  CAS  PubMed  Google Scholar 

  9. Till BJ, Burtner C, Comai L, Henikoff S (2004) Mismatch cleavage by single-strand specific nucleases. Nucleic Acids Res 32:2632–2641

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Dong C, Yu B (2011) Mutation surveyor: an in silico tool for sequencing analysis. In: Yu B, Hinchcliffe MJ (eds) Methods in molecular biology: in silico tools for gene discovery. Humana, Totowa, NJ, doi: 10.1007/978-1-61779-176-5_14

    Google Scholar 

  11. Greene EA, Codomo CA, Taylor NE, Henikoff JG, Till BJ, Reynolds SH, Enns LC, Burtner C, Johnson JE, Odden AR (2003) Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 164:731–740

    CAS  PubMed Central  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Chongmei Dong .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this protocol

Cite this protocol

Sharp, P., Dong, C. (2014). TILLING for Plant Breeding. In: Fleury, D., Whitford, R. (eds) Crop Breeding. Methods in Molecular Biology, vol 1145. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0446-4_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-0446-4_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0445-7

  • Online ISBN: 978-1-4939-0446-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics