Abstract
TILLING is widely used in plant functional genomics. Mutagenesis and SNP detection is combined to allow for the isolation of mutations in genes of interest. It can also be used as a plant breeding tool, whereby variation in known or candidate genes of interest to breeding programs is generated. Here we describe a simple low-cost TILLING procedure.
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References
McCallum CM, Comai L, Greene EA, Henikoff S (2000) Targeted screening for induced mutations. Nat Biotechnol 18:455–457
McCallum CM, Comai L, Greene EA, Henikoff S (2000) Targeting induced local lesions in genomes (TILLING) for plant functional genomics. Plant Physiol 123:439–442
Colbert T, Till BJ, Tompa R, Reynolds S, Steine MN, Yeung AT, McCallum CM, Comai L, Henikoff S (2001) High-throughput screening for induced point mutations. Plant Physiol 126:480–484
Wang T, Uauy C, Till B, Liu C-M (2010) TILLING and associated technologies. J Integr Plant Biol 52:2017–2030
Uauy C, Paraiso F, Colasuonno P, Tran RK, Tsai H, Berardi S, Comai L, Dubcovsky J (2009) A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat. BMC Plant Biol. doi:10.1186/1471-2229-9-115
Dong C, Vincent K, Sharp P (2009) Simultaneous mutation detection of three homoeologous genes in wheat by High Resolution Melting analysis and Mutation Surveyor®. BMC Plant Biol. doi:10.1186/1471-2229-9-143
Dong C, Dalton-Morgan C, Vincent K, Sharp P (2009) A modified TILLING method for wheat breeding. Plant Genome 2:39–47
Slade AJ, Fuerstenberg SI, Loeffler D, Steine MN, Facciotti D (2005) A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nat Biotechnol 23:75–81
Till BJ, Burtner C, Comai L, Henikoff S (2004) Mismatch cleavage by single-strand specific nucleases. Nucleic Acids Res 32:2632–2641
Dong C, Yu B (2011) Mutation surveyor: an in silico tool for sequencing analysis. In: Yu B, Hinchcliffe MJ (eds) Methods in molecular biology: in silico tools for gene discovery. Humana, Totowa, NJ, doi: 10.1007/978-1-61779-176-5_14
Greene EA, Codomo CA, Taylor NE, Henikoff JG, Till BJ, Reynolds SH, Enns LC, Burtner C, Johnson JE, Odden AR (2003) Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 164:731–740
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Sharp, P., Dong, C. (2014). TILLING for Plant Breeding. In: Fleury, D., Whitford, R. (eds) Crop Breeding. Methods in Molecular Biology, vol 1145. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0446-4_13
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DOI: https://doi.org/10.1007/978-1-4939-0446-4_13
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Online ISBN: 978-1-4939-0446-4
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