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Detection and Measurement of Paracaspase MALT1 Activity

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Caspases,Paracaspases, and Metacaspases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1133))

Abstract

The paracaspase MALT1 is a Cys-dependent, Arg-specific protease that plays an essential role in the activation and proliferation of lymphocytes during the immune response. Oncogenic activation of MALT1 is associated with the development of specific forms of B-cell lymphomas. Through specific cleavage of its substrates, MALT1 controls various aspects of lymphocyte activation, including the activation of transcriptional pathways, the stabilization of mRNAs, and an increase in cellular adhesion. In lymphocytes, the activity of MALT1 is tightly controlled by its inducible monoubiquitination, which promotes the dimerization of MALT1. Here, we describe both in vitro and in vivo assays that have been developed to assess MALT1 activity.

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Acknowledgments

We thank Katrin Cabalzar, Maike Jaworski, and Chantal Decaillet for critical reading of the manuscript. Work in the Thome laboratory is supported by the Swiss National Science Foundation, the Swiss Cancer League, the foundations Leenaards and Helmut Horten, the Novartis Foundation for Medical-Biological Research, and a collaboration agreement with Ono Pharmaceuticals.

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Authors and Affiliations

  1. Department of Biochemistry, University of Lausanne, Lausanne, Switzerland

    Stephan Hailfinger, Christiane Pelzer & Margot Thome

Authors
  1. Stephan Hailfinger
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  2. Christiane Pelzer
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  3. Margot Thome
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Editors and Affiliations

  1. Dept of Plant Biology & Forest Genetics, Swedish Univ of Agricultural Sciences Linnean Center for Plant Biology, Uppsala, Sweden

    Peter V. Bozhkov

  2. Sanford-Burnham Medical Research Inst. Apoptosis & Cell Death Research Prgm, La Jolla, California, USA

    Guy Salvesen

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Hailfinger, S., Pelzer, C., Thome, M. (2014). Detection and Measurement of Paracaspase MALT1 Activity. In: V. Bozhkov, P., Salvesen, G. (eds) Caspases,Paracaspases, and Metacaspases. Methods in Molecular Biology, vol 1133. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0357-3_11

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  • DOI: https://doi.org/10.1007/978-1-4939-0357-3_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0356-6

  • Online ISBN: 978-1-4939-0357-3

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