Abstract
Factors that influence the orientation of the mitotic spindle are important for the maintenance of stem cell populations and in cancer development. However, screening for these factors requires rapid quantification of alterations of the angle of the mitotic spindle in cultured cell lines. Here we describe a method to image mitotic cells and rapidly score the angle of the mitotic spindle using a simple MATLAB application to analyze a stack of Z-images.
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References
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Acknowledgement
This work was supported by NIH grant GM69429 to L.W.
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Decarreau, J., Driver, J., Asbury, C., Wordeman, L. (2014). Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells. In: Sharp, D. (eds) Mitosis. Methods in Molecular Biology, vol 1136. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0329-0_2
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DOI: https://doi.org/10.1007/978-1-4939-0329-0_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0328-3
Online ISBN: 978-1-4939-0329-0
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