Abstract
Ovules are the major female reproductive organs in higher plants. Furthermore, ovules of Arabidopsis thaliana are successfully used as model system to study plant organogenesis. Here we describe two microscopic techniques to analyze ovule development in Arabidopsis. Both methods involve fixed specimens and allow rapid, easy, and reproducible morphological comparisons between wild-type and mutant ovule development.
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References
Chevalier D, Sieber P, Schneitz K (2002) The genetic and molecular control of ovule development. In: O' Neill SD, Roberts JA (eds) Plant Reproduction. Sheffield Academic Press, Sheffield
Colombo L, Battaglia R, Kater MM (2008) Arabidopsis ovule development and its evolutionary conservation. Trends Plant Sci 13:444–450
Kelley DR, Gasser CS (2009) Ovule development: genetic trends and evolutionary considerations. Sex Plant Reprod 22:229–234
Shi D, Yang W (2011) Ovule development in Arabidopsis: progress and challenge. Curr Opin Plant Biol 14:74–80
Enugutti B, Kirchhelle C, Schneitz K (2012) On the genetic control of planar growth during tissue morphogenesis in plants. Protoplasma 250:651–661
Schneitz K, Hülskamp M, Pruitt RE (1995) Wild-type ovule development in Arabidopsis thaliana: a light microscope study of cleared whole-mount tissue. Plant J 7:731–749
Haseloff J (2003) Old botanical techniques for new microscopes. Biotechniques 34:1174–1182
Truernit E, Bauby H, Dubreucq B, Grandjean O, Runions J, Barthelemy J, Palauqui JC (2008) High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of phloem development and structure in Arabidopsis. Plant Cell 20:1494–1503
Christensen CA, King EJ, Jordan JR, Drews GN (1997) Megagametogenesis in Arabidopsis wild type and the Gf mutant. Sex Plant Reprod 10:49–64
Running MP, Clark SE, Meyerowitz EM (1995) Confocal microscopy of the shoot apex. Methods Cell Biol 49:217–229
Enugutti B, Oelschner M, Schneitz K (2013) Microscopic analysis of ovule development in Arabidopsis thaliana. Methods Mol Biol 959:127–136
Schneitz K, Hülskamp M, Kopczak SD, Pruitt RE (1997) Dissection of sexual organ ontogenesis: a genetic analysis of ovule development in Arabidopsis thaliana. Development 124:1367–1376
Smyth DR, Bowman JL, Meyerowitz EM (1990) Early flower development in Arabidopsis. Plant Cell 2:755–767
Acknowledgment
Our work is supported by grants from the Deutsche Forschungsgemeinschaft (German Research Council) to Kay Schneitz.
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Enugutti, B., Schneitz, K. (2014). Microscopic Analysis of Arabidopsis Ovules. In: Riechmann, J., Wellmer, F. (eds) Flower Development. Methods in Molecular Biology, vol 1110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9408-9_12
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DOI: https://doi.org/10.1007/978-1-4614-9408-9_12
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4614-9408-9
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