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Evaluation of Skin Damage Under UV Exposure

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Rheumatoid Arthritis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2766))

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Abstract

Photosensitivity disorder caused by sunlight, including ultraviolet (UV) rays, often occurs in connective tissue diseases such as lupus erythematosus. In addition, UVA (320–400 nM) and UVB (280–320 nM) trigger the progression of skin inflammation in the patients. Therefore, it is crucial to evaluate skin damage under UV exposure using experimental animals to clarify the relationship between connective tissue disease and photosensitivity disorder. In this chapter, our original protocol for evaluating UVA-dependent skin damage, which is known as photoaging via oxidative stress, is described.

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References

  1. Estadt SN, Maz MP, Musai J et al (2022) Mechanisms of photosensitivity in autoimmunity. J Invest Dermatol 142(special issue on autoimmunity):849–856. https://doi.org/10.1016/j.jid.2021.05.007

    Article  CAS  PubMed  Google Scholar 

  2. Pedić L, Pondeljak N, Šitum M (2020) Recent information on photoaging mechanisms and the preventive role of topical sunscreen products. Acta Dermatovenerol Alp Pannonica Adriat 29:201–207

    PubMed  Google Scholar 

  3. Krutmann J, Schroeder P (2009) Role of mitochondria in photoaging of human skin: the defective powerhouse model. J Investig Dermatol Symp Proc 14:44–49. https://doi.org/10.1038/jidsymp.2009.1

    Article  CAS  PubMed  Google Scholar 

  4. Cadet J, Sage E, Douki T (2005) Ultraviolet radiation-mediated damage to cellular DNA. Mutat Res 571:3–17. https://doi.org/10.1016/j.mrfmmm.2004.09.012

    Article  CAS  PubMed  Google Scholar 

  5. Cadet J, Douki T, Ravanat J-L (2008) Oxidatively generated damage to the guanine moiety of DNA: mechanistic aspects and formation in cells [WWW Document]. ACS Publishing. https://doi.org/10.1021/ar700245e

  6. Rinnerthaler M, Bischof J, Streubel MK et al (2015) Oxidative stress in aging human skin. Biomol Ther 5:545–589. https://doi.org/10.3390/biom5020545

    Article  CAS  Google Scholar 

  7. Chen J, Liu Y, Zhao Z et al (2021) Oxidative stress in the skin: impact and related protection. Int J Cosmet Sci 43:495–509. https://doi.org/10.1111/ics.12728

    Article  CAS  PubMed  Google Scholar 

  8. Abe YS, Sasaki S (2019) The adduct formation between the thioguanine-polyamine ligands and DNA with the AP site under UVA irradiated and non-irradiated conditions. Bioorg Med Chem 27:115160. https://doi.org/10.1016/j.bmc.2019.115160

    Article  CAS  PubMed  Google Scholar 

  9. Asaeda A, Ide H, Tano K et al (1998) Repair kinetics of abasic sites in mammalian cells selectively monitored by the aldehyde reactive probe (ARP). Nucleosides Nucleotides 17. https://doi.org/10.1080/07328319808005194

  10. Cadet J, Wagner JR (2013) DNA base damage by reactive oxygen species, oxidizing agents, and UV radiation. Cold Spring Harb Perspect Biol 5:a012559. https://doi.org/10.1101/cshperspect.a012559

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Cadet J, Douki T, Ravanat J-L (2015) Oxidatively generated damage to cellular DNA by UVB and UVA radiation. Photochem Photobiol 91:140–155. https://doi.org/10.1111/php.12368

    Article  CAS  PubMed  Google Scholar 

  12. Kasai H (1997) Analysis of a form of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat Res 387:147–163. https://doi.org/10.1016/S1383-5742(97)00035-5

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Takeshi Kiyoi .

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kiyoi, T. (2024). Evaluation of Skin Damage Under UV Exposure. In: Liu, S. (eds) Rheumatoid Arthritis. Methods in Molecular Biology, vol 2766. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3682-4_29

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  • DOI: https://doi.org/10.1007/978-1-0716-3682-4_29

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3681-7

  • Online ISBN: 978-1-0716-3682-4

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