Abstract
Bone homeostasis depends on the balance between bone deposition and bone resorption, which are mediated by the activity of osteoblasts and osteoclasts, respectively. Blocking osteoclast activity can be a therapeutic strategy in rheumatoid arthritis (RA) to reduce subsequent bone erosion. Therefore, investigating the activity of osteoclasts is essential for understanding the pathology of RA. Bone-resorption pits, which are caused by activated osteoclasts, are significantly increased in RA. Scanning electron microscopic analysis of bone-resorption pits is an effective method for understanding the pathology of RA. This chapter describes the method for observing the surface microstructure of pit formation on bone slices.
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Kiyoi, T. (2024). Scanning Electron Microscopic Analysis of the Bone-Resorption Activity in Mature Osteoclasts. In: Liu, S. (eds) Rheumatoid Arthritis. Methods in Molecular Biology, vol 2766. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3682-4_27
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DOI: https://doi.org/10.1007/978-1-0716-3682-4_27
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3682-4
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