Abstract
Mucin, a major component of the mucus, is considered to be one of the principal factors in the physiological defense mechanism of the gastrointestinal mucosa. Measuring the mucin content of human gastric mucus is a useful tool for the assessment of Helicobacter pylori (H. pylori) eradication or the involvement of mucus secretion in various gastroduodenal diseases. Here, we describe a methodology for the isolation of the mucin fraction from human gastric juice and the quantification of mucin.
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Acknowledgments
The authors express our sincere appreciation to S. Sugawara for her technical assistance.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Kawashima, R., Tsubokawa, D., Iijima, K., Ichikawa, T. (2024). Extraction and Fractionation of Human Gastric Mucins from Gastric Juice. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_5
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DOI: https://doi.org/10.1007/978-1-0716-3670-1_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3669-5
Online ISBN: 978-1-0716-3670-1
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