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DNA Barcoding in Species Delimitation: From Genetic Distances to Integrative Taxonomy

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DNA Barcoding

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2744))

Abstract

Over the past two decades, DNA barcoding has become the most popular exploration approach in molecular taxonomy, whether for identification, discovery, delimitation, or description of species. The present contribution focuses on the utility of DNA barcoding for taxonomic research activities related to species delimitation, emphasizing the following aspects:

(1) To what extent DNA barcoding can be a valuable ally for fundamental taxonomic research, (2) its methodological and theoretical limitations, (3) the conceptual background and practical use of pairwise distances between DNA barcode sequences in taxonomy, and (4) the different ways in which DNA barcoding can be combined with complementary means of investigation within a broader integrative framework. In this chapter, we recall and discuss the key conceptual advances that have led to the so-called renaissance of taxonomy, elaborate a detailed glossary for the terms specific to this discipline (see Glossary in Chap. 35), and propose a newly designed step-by-step species delimitation protocol starting from DNA barcode data that includes steps from the preliminary elaboration of an optimal sampling strategy to the final decision-making process which potentially leads to nomenclatural changes.

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Authors and Affiliations

  1. Department of Evolutionary Biology, Zoological Institute, Technische Universität Braunschweig, Braunschweig, Germany

    Aurélien Miralles & Miguel Vences

  2. Institut de Systématique, Évolution, Biodiversité (ISYEB), Muséum national d’Histoire naturelle, CNRS, Sorbonne Université, EPHE, Paris, France

    Aurélien Miralles & Nicolas Puillandre

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  1. Aurélien Miralles
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  2. Nicolas Puillandre
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  3. Miguel Vences
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Correspondence to Miguel Vences .

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Editors and Affiliations

  1. Division of Invertebrate Zoology, American Museum of Natural History, New York, NY, USA

    Robert DeSalle

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Miralles, A., Puillandre, N., Vences, M. (2024). DNA Barcoding in Species Delimitation: From Genetic Distances to Integrative Taxonomy. In: DeSalle, R. (eds) DNA Barcoding. Methods in Molecular Biology, vol 2744. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3581-0_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3581-0_4

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