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Validating a Mitochondrial Sweep Accompanying the Rapid Spread of a Maternally Inherited Symbiont

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Wolbachia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2739))

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Abstract

Maternally inherited symbiotic bacteria that interfere with the reproduction of their hosts can contribute to selective sweeps of mitochondrial haplotypes through hitch-hiking or coordinate inheritance of cytoplasmic bacteria and host mitochondria. The sweep will be manifested by genetic variations of mitochondrial genomic DNA of symbiont-infected hosts relative to their uninfected counterparts. In particular, at the population level, infected specimens will show a reduced mitochondrial DNA polymorphism compared to that in the nuclear DNA. This may challenge the use of mitochondrial DNA sequences as neutral genetic markers, as the mitochondrial patterns will reflect the evolutionary history of parasitism, rather than the sole evolutionary history of the host. Here, I describe a detailed step-by-step procedure to infer the occurrence and timing of symbiont-induced mitochondrial sweeps in host species.

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Acknowledgments

AD is funded by the Academy of Finland (grant #321543) and a HiLIFE fellowship. Thanks to the editor Prof. A. Fallon, as well as to Dr. P. Seppä, Dr. F. Valerio and Dr. V. Twort for comments on the manuscript.

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Authors and Affiliations

  1. Insect Symbiosis Ecology and Evolution, Organismal and Evolutionary Biology Research Program, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland

    Anne Duplouy

  2. Research Centre for Ecological Changes, University of Helsinki, Helsinki, Finland

    Anne Duplouy

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  1. Anne Duplouy
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Correspondence to Anne Duplouy .

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Editors and Affiliations

  1. Department of Entomology, University of Minnesota, Saint Paul, MN, USA

    Ann M. Fallon

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Duplouy, A. (2024). Validating a Mitochondrial Sweep Accompanying the Rapid Spread of a Maternally Inherited Symbiont. In: Fallon, A.M. (eds) Wolbachia. Methods in Molecular Biology, vol 2739. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3553-7_15

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  • DOI: https://doi.org/10.1007/978-1-0716-3553-7_15

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3552-0

  • Online ISBN: 978-1-0716-3553-7

  • eBook Packages: Springer Protocols

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