Abstract
Registration of larval zebrafish brain scans to a common reference brain enables comparison of transgene and gene expression patterns, neuroanatomy, and morphometry. Here we describe methods for staining and mounting larval zebrafish to facilitate whole-brain fluorescence imaging. Following image acquisition, we provide a template for aligning brain images to a reference atlas using nonlinear registration with the ANTs software package.
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Acknowledgments
This work was supported by the Intramural Research Program of the Eunice Kennedy Shriver National Institute for Child Health and Human Development (NICHD) and utilized the high-performance computational capabilities of the Biowulf Linux cluster at the National Institutes of Health, Bethesda, MD.
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Bhandiwad, A.A., Gupta, T., Subedi, A., Heigh, V., Holmes, G.A., Burgess, H.A. (2024). Brain Imaging and Registration in Larval Zebrafish. In: Amatruda, J.F., Houart, C., Kawakami, K., Poss, K.D. (eds) Zebrafish. Methods in Molecular Biology, vol 2707. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3401-1_9
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DOI: https://doi.org/10.1007/978-1-0716-3401-1_9
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