Abstract
Small teleost fishes such as zebrafish and medaka show remarkable regeneration capabilities upon tissue injury or amputation. To elucidate cellular mechanisms of teleost tissue repair and regeneration processes, the Cre/LoxP recombination system for cell lineage tracing is a widely used technique. In this chapter, we describe protocols used for inducible Cre/LoxP recombination-mediated lineage tracing of osteoblast progenitors during medaka fin regeneration as well as during the repair of osteoporosis-like bone lesions in the medaka vertebral column. Our approach can be adapted for lineage tracing of other cell populations in the regenerating teleost fin or in other tissues undergoing repair.
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Acknowledgments
We thank Lazaro Centanin (Centre for Organismal Studies, University of Heidelberg) for sharing the GaudiBBW2.1 transgenic line. We also thank the Centre for Bioimaging Sciences confocal unit and the fish facility at Department of Biological Sciences, National University of Singapore, for continued support. This work was supported by the Singapore Ministry of Education (MOE2016-T2-2-086) and the National Research Foundation Singapore (NRF2017-NRF-ISF002-2671). The authors declare no competing interests.
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Tan, W.H., Winkler, C. (2024). Lineage Tracing of Bone Cells in the Regenerating Fin and During Repair of Bone Lesions. In: Amatruda, J.F., Houart, C., Kawakami, K., Poss, K.D. (eds) Zebrafish. Methods in Molecular Biology, vol 2707. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3401-1_6
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DOI: https://doi.org/10.1007/978-1-0716-3401-1_6
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