Abstract
Zebrafish-based high-throughput screening has been extensively used to study toxicological profiles of individual chemicals and mixtures, identify novel toxicants, and study modes of action to prioritize chemicals for further testing and policy decisions. Within this chapter, we describe a protocol for automated zebrafish developmental high-throughput screening in our laboratory, with emphasis on exposure setups, morphological and behavioral readouts, and quality control.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Truong L, Tanguay RL (2017) Evaluation of embryotoxicity using the zebrafish model. In: Gautier J-C (ed) Drug safety evaluation: methods and protocols. Springer, New York, pp 325–333. https://doi.org/10.1007/978-1-4939-7172-5_18
Truong L, Tanguay RL (2017) Evaluation of embryotoxicity using the zebrafish model. Methods Mol Biol 1641:325–333. https://doi.org/10.1007/978-1-4939-7172-5_18
Garcia GR, Noyes PD, Tanguay RL (2016) Advancements in zebrafish applications for 21st century toxicology. Pharmacol Ther 161:11–21
OECD (2019) Test no. 203: fish, acute toxicity test. https://doi.org/10.1787/9789264069961-en
Mandrell D, Truong L, Jephson C et al (2012) Automated zebrafish chorion removal and single embryo placement: optimizing throughput of zebrafish developmental toxicity screens. J Lab Autom 17(1):66–74. https://doi.org/10.1177/2211068211432197
Barton CL, Johnson EW, Tanguay RL (2016) Facility design and health management program at the Sinnhuber Aquatic Research Laboratory. Zebrafish 13(Suppl 1):S39–S43. https://doi.org/10.1089/zeb.2015.1232
Kimmel CB, Ballard WW, Kimmel SR et al (1995) Stages of embryonic development of the zebrafish. Dev Dyn 203(3):253–310. https://doi.org/10.1002/aja.1002030302
Truong L, Reif DM, St Mary L et al (2014) Multidimensional in vivo hazard assessment using zebrafish. Toxicol Sci 137(1):212–233. https://doi.org/10.1093/toxsci/kft235
Reif DM, Truong L, Mandrell D et al (2016) High-throughput characterization of chemical-associated embryonic behavioral changes predicts teratogenic outcomes. Arch Toxicol 90(6):1459–1470. https://doi.org/10.1007/s00204-015-1554-1
Truong L, Marvel S, Reif DM et al (2020) The multi-dimensional embryonic zebrafish platform predicts flame retardant bioactivity. Reprod Toxicol 96:359–369. https://doi.org/10.1016/j.reprotox.2020.08.007
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Dasgupta, S., Simonich, M.T., Tanguay, R.L. (2024). Developmental Toxicity Assessment Using Zebrafish-Based High-Throughput Screening. In: Amatruda, J.F., Houart, C., Kawakami, K., Poss, K.D. (eds) Zebrafish. Methods in Molecular Biology, vol 2707. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3401-1_4
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3401-1_4
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3400-4
Online ISBN: 978-1-0716-3401-1
eBook Packages: Springer Protocols