Abstract
Phage display is a technique that allows the presentation of unique proteins on the surface of bacteriophages. The phage particles are usually screened via repetitive rounds of antigen-guided selection and phage amplification. The main advantage of this approach lies in the physical linkage between phenotype and genotype. This feature allows the isolation of single unique clones from a panning campaign consisting of a highly diverse population of clones. Due to the high-throughput nature of this technique, different approaches have been developed to assist phage display selections. One of which involves utilizing a streptavidin-coated solid-phase extraction (SPE) tip that is mounted to an electronically controlled motorized multichannel pipette. In this chapter, we will entail the procedures involved in the adaptation of a commercial SPE tip (MSIA™ streptavidin D.A.R.T’s®) as the solid phase. This protocol is an updated version of a previous protocol with some minor refinements.
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Acknowledgments
The authors would like to acknowledge the support of the Malaysian Ministry of Higher Education Fundamental Research Grant Scheme [FRGS/1/2018/STG05/USM/02/2 – (203/CIPPM/6711658)].
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Lim, T.S., Ch’ng, A.C.W., Song, B.P.C., Lai, J.Y. (2023). Streptavidin-Coated Solid-Phase Extraction (SPE) Tips for Antibody Phage Display Biopanning. In: Hust, M., Lim, T.S. (eds) Phage Display. Methods in Molecular Biology, vol 2702. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3381-6_14
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DOI: https://doi.org/10.1007/978-1-0716-3381-6_14
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