Abstract
CD4 T-cells are important for long-term control and clearance of several fungal infections in humans, particularly those caused by Cryptococcus species. Understanding the mechanisms underlying protective T-cell immunity against fungal infection is critical for developing mechanistic insights into the pathogenesis of the disease. Here, we describe a protocol that enables analysis of fungal-specific CD4 T-cell responses in vivo, using adoptive transfer of fungal-specific T-cell receptor (TCR) transgenic CD4 T-cells. While the protocol here uses a TCR transgenic model reactive to peptide deriving from Cryptococcus neoformans, this method could be adapted to other fungal infection experimental settings.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Fu, M.S., Kawakami, K., Drummond, R.A. (2023). Adoptive Transfer of Cryptococcus neoformans-Specific CD4 T-Cells to Study Anti-fungal Lymphocyte Responses In Vivo. In: Drummond, R.A. (eds) Antifungal Immunity. Methods in Molecular Biology, vol 2667. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3199-7_7
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DOI: https://doi.org/10.1007/978-1-0716-3199-7_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3198-0
Online ISBN: 978-1-0716-3199-7
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