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Carbohydrate Depolymerization by Intricate Cellulosomal Systems

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Carbohydrate-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2657))

Abstract

Cellulosomes are multi-enzymatic nanomachines that have been fine-tuned through evolution to efficiently deconstruct plant biomass. Integration of cellulosomal components occurs via highly ordered protein-protein interactions between the various enzyme-borne dockerin modules and the multiple copies of the cohesin modules located on the scaffoldin subunit. Recently, designer cellulosome technology was established to provide insights into the architectural role of catalytic (enzymatic) and structural (scaffoldin) cellulosomal constituents for the efficient degradation of plant cell wall polysaccharides. Owing to advances in genomics and proteomics, highly structured cellulosome complexes have recently been unraveled, and the information gained has inspired the development of designer-cellulosome technology to new levels of complex organization. These higher-order designer cellulosomes have in turn fostered our capacity to enhance the catalytic potential of artificial cellulolytic complexes. In this chapter, methods to produce and employ such intricate cellulosomal complexes are reported.

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Author information

Authors and Affiliations

  1. Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

    Sarah Moraïs, Edward A. Bayer & Itzhak Mizrahi

  2. Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot, Israel

    Johanna Stern, Lior Artzi & Edward A. Bayer

  3. NZYTech Genes & Enzymes, Lisbon, Portugal

    Carlos M. G. A. Fontes

Authors
  1. Sarah Moraïs
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  2. Johanna Stern
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  3. Lior Artzi
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  4. Carlos M. G. A. Fontes
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  5. Edward A. Bayer
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  6. Itzhak Mizrahi
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Corresponding author

Correspondence to Edward A. Bayer .

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Editors and Affiliations

  1. Agriculture and Agri-Food Canada, Lethbridge Research and Development Center, Lethbridge, AB, Canada

    D. Wade Abbott

  2. Department of Biochemistry, The University of British Columbia, Kelowna, BC, Canada

    Wesley F. Zandberg

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Moraïs, S., Stern, J., Artzi, L., Fontes, C.M.G.A., Bayer, E.A., Mizrahi, I. (2023). Carbohydrate Depolymerization by Intricate Cellulosomal Systems. In: Abbott, D.W., Zandberg, W.F. (eds) Carbohydrate-Protein Interactions. Methods in Molecular Biology, vol 2657. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3151-5_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3151-5_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3150-8

  • Online ISBN: 978-1-0716-3151-5

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