Abstract
We present an integrated droplet-based microfluidic platform for high-throughput protein crystallization experimentation. The device consists of commercially available micro-junctions and PFA tubing assembled for different functions: droplet generation, incubation, and observation. Herein, we describe a complete methodology to generate hundreds of droplets with controlled properties (i.e., size, generation frequency, and composition). Therefore, multiple trials can be carried out under controlled experimental conditions for the screening and optimization of protein crystallization conditions.
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Acknowledgments
J. F. acknowledges funding from CEFT under FCT/MCTES (PIDDAC) through a postdoctoral scholarship. This work was financially supported by: HealthyWaters (NORTE-01-0145-FEDER-000069), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); and LA/P/0045/2020 (ALiCE), UIDB/00532/2020 and UIDP/00532/2020 (CEFT), UIDB/00511/2020 and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC).
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Ferreira, J., Castro, F. (2023). High-Throughput Protein Crystallization in an Integrated Droplet-Based Microfluidic Platform. In: Sousa, Â., Passarinha, L. (eds) Advanced Methods in Structural Biology. Methods in Molecular Biology, vol 2652. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3147-8_20
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DOI: https://doi.org/10.1007/978-1-0716-3147-8_20
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