Abstract
The left-handed Z-DNA is surrounded by right-handed canonical B-DNA, and thus the junction between B- and Z-DNA has been occurred during temporal Z-DNA formation in the genome. The base extrusion structure of the BZ junction may help detect Z-DNA formation in DNAs. Here we describe the BZ junction structural detection by using 2-aminopurine (2AP) fluorescent probe. BZ junction formation can be measured in solution by this method.
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Acknowledgments
This work was supported by National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science, and Technology (MSIT) of the Korean government (2020RC1C1C1007371 to D.K.).
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Kang, M., Kim, D. (2023). BZ Junctions and Its Application as Probe (2AP) to Detect Z-DNA Formation and Its Effector. In: Kim, K.K., Subramani, V.K. (eds) Z-DNA. Methods in Molecular Biology, vol 2651. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3084-6_7
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DOI: https://doi.org/10.1007/978-1-0716-3084-6_7
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