Abstract
Generating genetically modified animal models that precisely recapitulate disease characteristics forms an integral and indispensable tool to understanding disease pathophysiology. Recently, important advances in genome editing technologies have enabled us to efficiently create sophisticated animal models in short periods of time. Base editing is a modified CRISPR/Cas system that induces base substitution at targeted genomic regions. Here I describe a basic protocol to introduce disease-relevant pathogenic mutations into mice utilizing two representative base editing tools, Base Editor and Target-AID.
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References
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Acknowledgments
I thank Professor Toru Takeo (CARD, Kumamoto University) for providing valuable protocols of mouse embryo manipulations. This work was supported by AMED under Grant Number JP15dm0207001 (Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS)) and JSPS KAKENHI Grant Number JP18K07402.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Sasaguri, H. (2023). Introduction of Genetic Mutations Into Mice by Base Editor and Target-AID. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 2637. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3016-7_9
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DOI: https://doi.org/10.1007/978-1-0716-3016-7_9
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