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Genome Editing of Pig

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Genome Editing in Animals

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2637))

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Abstract

Pigs have anatomical and physiological characteristics similar to humans; therefore, genetically modified pigs have the potential to become a valuable bioresource in biomedical research. In fact, considering the increasing need for translational research, pigs are useful for studying intractable diseases, organ transplantation, and regenerative medicine as large-scale experimental animals with excellent potential for extrapolation to humans. With the advent of zinc finger nucleases (ZFNs), breakthroughs in genome editing tools such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) have facilitated the efficient generation of genetically modified pigs. Genome editing has been used in pigs for more than 10 years; now, along with knockout pigs, knock-in pigs are also gaining increasing importance. In this chapter, we describe the establishment of gene-modified cells (nuclear donor cells), which are necessary for gene knockout and production of knock-in pigs via somatic cell nuclear transplantation, as well as the production of gene knockout pigs using a simple cytoplasmic injection method.

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Acknowledgment

This work was supported by a Grant-in-Aid for Scientific Research (A) from the Japan Society for the Promotion of Science (No. 15H02480 to Hiroshi Nagashima), the Meiji University International Institute for Bio-Resource Research (MUIIBR to Hiroshi Nagashima), the Nakauchi Stem Cell and Organ Regeneration Project, the Exploratory Research for Advanced Technology (ERATO), the Japan Science and Technology Agency (JST), the Leading Advanced Projects for Medical Innovation (LEAP), and the Advanced Research and Development Programs for Medical Innovation (AMED).

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Author notes

  1. Masahito Watanabe

    Present address: PorMedTec Co., Ltd., Kawasaki, Kanagawa, Japan

Authors and Affiliations

  1. Meiji University International Institute for Bio-Resource Research, Kawasaki, Kanagawa, Japan

    Masahito Watanabe & Hiroshi Nagashima

  2. Laboratory of Medical Bioengineering, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan

    Hiroshi Nagashima

Authors
  1. Masahito Watanabe
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  2. Hiroshi Nagashima
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Corresponding author

Correspondence to Hiroshi Nagashima .

Editor information

Editors and Affiliations

  1. Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan

    Izuho Hatada

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Watanabe, M., Nagashima, H. (2023). Genome Editing of Pig. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 2637. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3016-7_21

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  • DOI: https://doi.org/10.1007/978-1-0716-3016-7_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3015-0

  • Online ISBN: 978-1-0716-3016-7

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