Abstract
Rat germline-competent embryonic stem (ES) cell lines have been available since 2008, and rat models with targeted mutations have been successfully generated using ES cell-based genome targeting technology. This chapter will focus on the procedures of gene targeting in rat ES cells.
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References
Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292(5819):154–156
Capecchi MR (2005) Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Nat Rev Genet 6(6):507–512
Wu S et al (2007) Toward simpler and faster genome-wide mutagenesis in mice. Nat Genet 39(7):922–930
Buehr M et al (2008) Capture of authentic embryonic stem cells from rat blastocysts. Cell 135(7):1287–1298
Li P et al (2008) Germline competent embryonic stem cells derived from rat blastocysts. Cell 135(7):1299–1310
Tong C (2010) Production of p53 gene knockout rats by homologous recombination in embryonic stem cells. Nature 467(7312):211–213
Tong C et al (2011) Generating gene knockout rats by homologous recombination in embryonic stem cells. Nat Protoc 6(6):827–844
Nichols J, Ying QL (2006) Derivation and propagation of embryonic stem cells in serum- and feeder-free culture. Methods Mol Biol 329:91–98
Acknowledgements
This work was supported by a grant from National Institutes of Health: P40 OD011062 (ECB).
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Men, H., Davis, D.J., Bryda, E.C. (2023). Gene Targeting in Rat Embryonic Stem Cells. In: Saunders, T.L. (eds) Transgenesis. Methods in Molecular Biology, vol 2631. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2990-1_15
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DOI: https://doi.org/10.1007/978-1-0716-2990-1_15
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2990-1
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