Abstract
Whole-mount in situ hybridization (WISH) is a technique that enables temporal and spatial visualization of RNA molecules in an embryo or whole tissue by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20–25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in Xenopus laevis or tropicalis by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.
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Acknowledgements
We would like to thank the members of the Wheeler and Munsterberg labs, both past and present, who have contributed to the development of these protocols.
Funding
MA is funded by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 860635. AMG was supported by UKRI Biotechnology and Biological Sciences Research Council Norwich Research Park Biosciences Doctoral Training Partnership grants (BB/M011216/1).
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Antonaci, M., Godden, A.M., Wheeler, G.N. (2023). Determining miRNA Expression Patterns in Xenopus. In: Dalmay, T. (eds) MicroRNA Detection and Target Identification. Methods in Molecular Biology, vol 2630. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2982-6_11
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DOI: https://doi.org/10.1007/978-1-0716-2982-6_11
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