Abstract
Recent advances have revolutionized the oldest high-throughput single-cell analytical tool, flow cytometry. Fluorescent analyzers and sorters with up to seven lasers and the potential to detect up to 50 parameters are changing the way flow cytometry is used, but old school practices which are inadequate for new technologies remain alive. This chapter summarizes recent advances, explains the most salient new features and offers a step-by-step guide to develop and successfully execute high-dimensional fluorescent flow cytometry experiments.
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Acknowledgments
The authors wish to acknowledge Karen Wolcott (CCR/LGI Flow Core) for help with training on the Sony ID7000 spectral analyzer, Jeff Clapper (Sony Biotechnology) for helpful discussions and insight in spectral flow cytometry and Hidehiro Yamane, and Mariah Lee (CCR/LCMB, NCI, NIH) for providing unpublished data in Figs. 6 and 7 for the comparison of conventional and spectral flow cytometry analyses.
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Siddiqui, S., Livák, F. (2023). Principles of Advanced Flow Cytometry: A Practical Guide. In: Bosselut, R., Vacchio, M.S. (eds) T-Cell Development. Methods in Molecular Biology, vol 2580. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2740-2_5
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DOI: https://doi.org/10.1007/978-1-0716-2740-2_5
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