Abstract
Electroretinogram (ERG) captures the electrical responses of photoreceptors, the summation of action potentials from all neurons in the retina elicited by illumination. ERG testing is an incredibly useful tool in obtaining more specific information regarding a retinal dystrophy. Specifically, ERGs are typically used to test photoreceptors and inner retinal function in humans and animals, to diagnose retinal dystrophies, and to monitor disease progression. In this chapter, we will introduce the components of ERGs and the standard ERG protocols for clinical examination. We will also introduce the various specialized ERG tests, which can help to differentiate retinitis pigmentosa (RP) from other retinal disorders. Lastly, we will elaborate on how to use ERGs to predict visual prognosis in RP.
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References
Dewar J (1877) The physiological action of Light1. Nature 15(385):433–435
Kahn RH, Löwenstein A (1924) Das Elektroretinogramm. Albrecht von Graefes Archiv für Ophthalmologie 114(2):304–331
de-Rouck AF (2006) History of the electroretinogram. In: Heckenlively JR, Arden GB (eds) Principles and practice of clinical electrophysiology of vision. MIT Press, Cambridges, pp 3–10
Riggs LA (1941) Continuous and reproducible records of the electrical activity of the human retina. Proc Soc Exp Biol Med 48(1):204–207
Frishman LJ, Wang MH (2011) Electroretinogram of human, monkey and mouse. In: Levin LA et al (eds) Adler’s physiology of the eye. Elsevier Health Sciences, pp 480–501
Perlman I (2007) The electroretinogram: ERG. In: Kolb H, Fernandez E, Nelson R (eds) Webvision: the organization of the retina and visual system [Internet]. University of Utah Health Sciences Center, pp 1323–1364
Frishman LJ (2006) Origins of the electroretinogram. In: Heckenlively JR, Arden GB (eds) Principles and practice of clinical electrophysiology of vision. MIT Press, Cambridges, pp 139–184
Falk G, Shiells R (2006) Synaptic transmission: sensitivity control mechanisms. In: Heckenlively JR, Arden GB (eds) Principles and practice of clinical electrophysiology of vision. MIT Press, Cambridges, pp 79–92
Gurevich L, Slaughter MM (1993) Comparison of the waveforms of the ON bipolar neuron and the b-wave of the electroretinogram. Vis Res 33(17):2431–2435
Miller RF, Dowling JE (1970) Intracellular responses of the Muller (glial) cells of mudpuppy retina: their relation to b-wave of the electroretinogram. J Neurophysiol 33(3):323–341
Newman EA, Odette LL (1984) Model of electroretinogram b-wave generation: a test of the K+ hypothesis. J Neurophysiol 51(1):164–182
Stockton RA, Slaughter MM (1989) B-wave of the electroretinogram. A reflection of ON bipolar cell activity. J Gen Physiol 93(1):101–122
Coupland SG (2006) Electrodes for visual testing. In: Heckenlively JR, Arden GB (eds) Principles and practice of clinical electrophysiology of vision. MIT Press, Cambridges, pp 245–254
Burian HM, Allen L (1954) A speculum contact lens electrode for electroretinography. Electroencephalogr Clin Neurophysiol 6(3):509–511
McCulloch DL et al (2015) ISCEV standard for full-field clinical electroretinography (2015 update). Doc Ophthalmol 130(1):1–12
Lam BL (2005) Electrophysiology of vision: clinical testing and applications. CRC Press
Lawwill T, Burian HM (1966) A modification of the Burian-Allen contact-lens electrode for human electroretinography. Am J Ophthalmol 61(6):1506–1509
Robson AG et al (2018) ISCEV guide to visual electrodiagnostic procedures. Doc Ophthalmol 136(1):1–26
Marmor MF et al (2009) ISCEV standard for full-field clinical electroretinography (2008 update). Doc Ophthalmol 118(1):69–77
Berson EL (1981) Retinitis pigmentosa and allied diseases: applications of electroretinographic testing. Int Ophthalmol 4(1–2):7–22
Falk G (1988) Signal transmission from rods to bipolar and horizontal cells: a synthesis. Prog Retin Res 8:255–279
Wachtmeister L (1998) Oscillatory potentials in the retina: what do they reveal. Prog Retin Eye Res 17(4):485–521
Wang NK et al (2012) Multimodal fundus imaging in fundus albipunctatus with RDH5 mutation: a newly identified compound heterozygous mutation and review of the literature. Doc Ophthalmol 125(1):51–62
Wang NK et al (2009) Cellular origin of fundus autofluorescence in patients and mice with a defective NR2E3 gene. Br J Ophthalmol 93(9):1234–1240
Perlman I et al (2019) ISCEV extended protocol for the S-cone ERG. Doc Ophthalmol 140:95–101
Berson EL (1993) Retinitis pigmentosa. The Friedenwald Lecture. Invest Ophthalmol Vis Sci 34(5):1659–1676
Berson EL (2007) Long-term visual prognoses in patients with retinitis pigmentosa: the Ludwig von Sallmann lecture. Exp Eye Res 85(1):7–14
Nusinowitz S, Heckenlively JR (2006) Evaluating retinal function in the mouse retina with the electroretinogram. In: Heckenlively JR, Arden GB (eds) Principles and practice of clinical electrophysiology of vision. MIT Press, Cambridges, pp 899–910
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Huang, WC., Liu, PK., Wang, NK. (2023). Electroretinogram (ERG) to Evaluate the Retina in Cases of Retinitis Pigmentosa (RP). In: Tsang, S.H., Quinn, P.M. (eds) Retinitis Pigmentosa. Methods in Molecular Biology, vol 2560. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2651-1_10
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DOI: https://doi.org/10.1007/978-1-0716-2651-1_10
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