Abstract
Methods to synthesize influenza virus inhibitors with fluoro, phosphono, and/or sulfo functional groups are described. The resulting sialic acid analogues are produced from the natural substrate N-acetylneuraminic acid as starting material. Fluorescent assay methods for inhibition of influenza neuraminidase and virus proliferation are also provided.
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Abbreviations
- 4-MU:
-
4-Methylumbelliferone
- 4-MU-Gal:
-
4-Methylumbelliferyl β-d-galactoside
- Ab:
-
Antibody
- anhyd.:
-
Anhydrous
- BSC:
-
Biosafety cabinet
- DAST:
-
Diethylaminosulfur trifluoride
- E:
-
Enzyme
- e.g.:
-
For example
- Em:
-
Emission wavelength
- Ex:
-
Excitation wavelength
- Gal:
-
Galactose
- i.e.:
-
That is
- IAV :
-
Influenza A virus
- IC50:
-
Half-maximal inhibitory concentration
- MDCK:
-
Madin-Darby canine kidney
- MEM:
-
Minimum essential medium
- MES:
-
2-(N-Morpholino)ethanesulfonic acid
- MUNANA:
-
4-Methylumbelliferyl-Neu5Ac
- NA :
-
Neuraminidase
- Neu5Ac :
-
N-acetylneuraminic acid
- NP:
-
Nucleoprotein
- PBS:
-
Phosphate-buffered salinesat.: Saturated
- ST6Gal I:
-
β-Galactoside α(2–6)-sialyltransferase I soln.: Solution
- Tyr:
-
Tyrosine
References
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Vavricka, C.J., Sriwilaijaroen, N., Suzuki, Y., Kiyota, H. (2022). Synthesis and Neuraminidase Inhibitory Activity of Sialic Acid Analogues with Fluoro, Phosphono, and Sulfo Functionalities. In: Suzuki, Y. (eds) Glycovirology. Methods in Molecular Biology, vol 2556. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2635-1_20
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DOI: https://doi.org/10.1007/978-1-0716-2635-1_20
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