Abstract
Protein aggregates are a common feature of nearly all neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis (ALS). Here we describe a method to quickly and accurately measure protein aggregation in cells expressing a fluorescently tagged aggregation-prone protein. This unbiased method obviates the need for manual scoring and facilitates the identification of factors governing protein self-assembly and its downstream consequences for cell heath.
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Safren, N., Sharkey, L.M., Barmada, S.J. (2023). Neuronal Puncta/Aggregate Formation by WT and Mutant UBQLN2. In: Cieplak, A.S. (eds) Protein Aggregation. Methods in Molecular Biology, vol 2551. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2597-2_34
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DOI: https://doi.org/10.1007/978-1-0716-2597-2_34
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