Abstract
Sleep is an essential physiological behavior required for all animals to live. And sleep is one of important functional outputs of circadian rhythm. Long-term sleep deprivation results in severe health impairment or death. However, the molecular mechanisms and neuronal circuitry that regulate sleep remain unclear. Although animal activity (wakefulness) is usually monitored by infrared sensor or running wheel in study of circadian rhythm, analysis of sleep is also important to understand inactive period. Various animal models have been used to help understand regulatory mechanism of sleep. Among them, rodents are often used for sleep research due to their similarities in sleep architecture with humans, time to maturation, moderate body and brain size, as well as widely studied brain anatomy and genomic information (Suckow and Stewart KL. Principles of animal research for graduate and undergraduate students. Academic Press is an imprint of Elsevier, London/San Diego, CA, 2017). In this chapter, we describe detailed protocols for sleep studies in mice under freely moving conditions and discuss the pros and cons of these methods.
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Hung, C., Yamanaka, A. (2022). Assessment of Sleep in Mice. In: Hirota, T., Hatori, M., Panda, S. (eds) Circadian Clocks. Neuromethods, vol 186. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2577-4_13
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DOI: https://doi.org/10.1007/978-1-0716-2577-4_13
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