Abstract
Pseudomonas savastanoi is a phytopathogenic bacterium causing severe disease on olive, oleander, ash, and other Oleaceae. Three main pathovars belong to this species: P. savastanoi pv. savastanoi, pv. nerii, and pv. fraxini. Detection methods are mostly based on the visual inspection of the typical symptoms (i.e., knots and galls). However, this bacterium can survive on the host plant also as an epiphyte without giving any symptom. To avoid the spread of P. savastanoi to areas where it is absent, it is necessary to develop efficient and sensitive detection methods. Here, we reported three different PCR-based techniques, able to discriminate the three P. savastanoi pathovars attacking woody plants.
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Acknowledgment
This work was supported by INSTM with a grant to C.P. on a project coordinated by S.T.
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Tegli, S., Pastacaldi, C. (2022). Specific Detection of Pseudomonas savastanoi Pathovars: From End-Point PCR to Real-Time PCR and HRMA. In: Luchi, N. (eds) Plant Pathology. Methods in Molecular Biology, vol 2536. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2517-0_16
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DOI: https://doi.org/10.1007/978-1-0716-2517-0_16
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