Abstract
Measuring quantitative changes in plant hormones and derivatives is crucial to understand how reactive oxygen species trigger signaling cascades to regulate stress responses. In this chapter, we describe the liquid chromatography-mass spectrometry procedure that we use to extract and quantify salicylic acid (SA), jasmonic acid (JA), and related compounds in common extracts of Arabidopsis tissue. The method can provide quantitative data on SA, SA glucosides, and JA, as well as information on oxidized and conjugated forms of these compounds and related derivatives of benzoic acid.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Mhamdi A, Van Breusegem F (2018) Reactive oxygen species in plant development. Development 145
Noctor G, Reichheld JP, Foyer CH (2018) ROS-related signaling in plants. Semin Cell Dev Biol 80:3–12
Vanacker H, Carver TLW, Foyer CH (2000) Early H2O2 accumulation in mesophyll cells leads to induction of glutathione during the hypersensitive response in the barley-powdery mildew interaction. Plant Physiol 123:1289–1300
Laloi C, Mestres-Ortega MY, Meyer Y, Reichheld JP (2004) The Arabidopsis cytosolic thioredoxin h5 gene induction by oxidative stress and its W-box-mediated response to pathogen elicitor. Plant Physiol 134:1006–1016
Mou Z, Fan WH, Dong XN (2003) Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes. Cell 113:935–944
Mhamdi A, Hager J, Chaouch S et al (2010) Arabidopsis GLUTATHIONE REDUCTASE 1 is essential for the metabolism of intracellular H2O2 and to enable appropriate gene expression through both salicylic acid and jasmonic acid signaling pathways. Plant Physiol 153:1144–1160
Han Y, Chaouch S, Mhamdi A et al (2013) Functional analysis of Arabidopsis mutants points to novel roles for glutathione in coupling H2O2 to activation of salicylic acid accumulation and signaling. Antioxid Redox Signal 18:2106–2121
Han Y, Mhamdi A, Chaouch S, Noctor G (2013) Regulation of basal and oxidative stress-triggered jasmonic acid-related gene expression by glutathione. Plant Cell Environ 36:1135–1146
Dempsey DMA, Vlot AC, Wildermuth MC, Klessig DF (2011) Salicylic acid biosynthesis and metabolism. The Arabidopsis Book 9:e0156
Miersch O, Neumerkel J, Dippe M, Stenzel I, Wasternack C (2008) Hydroxylated jasmonates are commonly occurring metabolites of jasmonic acid and contribute to a partial switch-off in jasmonate signalling. New Phytol 177:114–127
Acosta IF, Farmer EE (2010) Jasmonates. The Arabidopsis Book 8:e0129
Bartsch M, Bednarek P, Vivancos PD et al (2010) Accumulation of isochorismate-derived 2,3-dihydroxybenzoic 3-O-β-d-xyloside in Arabidopsis resistance to pathogens and ageing of leaves. J Biol Chem 285:25654–25665
Le Roux C, Del Prete S, Boutet-Mercey S, Perreau F, Balagué C, Roby D, Fagard M, Gaudin V (2014) The hnRNP-Q protein LIF2 participates in the plant immune response. PLoS One 9:e99343
Acknowledgments
We thank Grégory Mouille (IJPB, INRA Versailles) for discussion. Work in the GN laboratory is supported by the French Agence Nationale de la Recherche HIPATH project (ANR-17-CE20-0025) and by the Institut Universitaire de France (IUF).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Lelarge-Trouverie, C., Mhamdi, A., Guérard, F., Noctor, G. (2022). Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds. In: Mhamdi, A. (eds) Reactive Oxygen Species in Plants. Methods in Molecular Biology, vol 2526. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2469-2_16
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2469-2_16
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2468-5
Online ISBN: 978-1-0716-2469-2
eBook Packages: Springer Protocols