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Sample Preparation, Data Acquisition, and Data Analysis for 15N-Labeled and Nonlabeled Monoterpene Indole Alkaloids in Catharanthus roseus

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Catharanthus roseus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2505))

Abstract

Recent approaches developed in metabolomics using liquid chromatography–tandem mass spectrometry (LC-MS/MS) enabled us to assign a part of specialized metabolites in plants. However, the approaches are not good enough for the rest of the metabolites, which are still unknown. To characterize the unknown metabolites, more appropriate and precise approaches need to be developed. Here, a procedure to analyze 15N-labeled and nonlabeled LC-MS/MS data for identification of monoterpene indole alkaloids was developed.

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Acknowledgments

I would like to thank Tetsuya Mori and Kazuki Saito (RIKEN CSRS) for technical assistance on LC-MS/MS and comments to the project, respectively.

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Authors and Affiliations

  1. RIKEN Center for Sustainable Resource Science, Yokohama, Japan

    Ryo Nakabayashi

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  1. Ryo Nakabayashi
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Correspondence to Ryo Nakabayashi .

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Editors and Affiliations

  1. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Vincent Courdavault

  2. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Sebastien Besseau

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Nakabayashi, R. (2022). Sample Preparation, Data Acquisition, and Data Analysis for 15N-Labeled and Nonlabeled Monoterpene Indole Alkaloids in Catharanthus roseus. In: Courdavault, V., Besseau, S. (eds) Catharanthus roseus. Methods in Molecular Biology, vol 2505. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2349-7_4

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  • DOI: https://doi.org/10.1007/978-1-0716-2349-7_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2348-0

  • Online ISBN: 978-1-0716-2349-7

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