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Solid-State NMR Spectroscopy on Microbial Rhodopsins

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Rhodopsin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2501))

Abstract

Microbial rhodopsins represent the most abundant phototrophic systems known today. A similar molecular architecture with seven transmembrane helices and a retinal cofactor linked to a lysine in helix 7 enables a wide range of functions including ion pumping, light-controlled ion channel gating, or sensing. Deciphering their molecular mechanisms therefore requires a combined consideration of structural, functional, and spectroscopic data in order to identify key factors determining their function. Important insight can be gained by solid-state NMR spectroscopy by which the large homo-oligomeric rhodopsin complexes can be studied directly within lipid bilayers. This chapter describes the methodological background and the necessary sample preparation requirements for the study of photointermediates, for the analysis of protonation states, H-bonding and chromophore conformations, for 3D structure determination, and for probing oligomer interfaces of microbial rhodopsins. The use of data extracted from these NMR experiments is discussed in the context of complementary biophysical methods.

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Authors and Affiliations

  1. Institute of Biophysical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany

    Clara Nassrin Kriebel, Johanna Becker-Baldus & Clemens Glaubitz

  2. Centre for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Frankfurt am Main, Germany

    Clara Nassrin Kriebel, Johanna Becker-Baldus & Clemens Glaubitz

Authors
  1. Clara Nassrin Kriebel
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  2. Johanna Becker-Baldus
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  3. Clemens Glaubitz
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Correspondence to Clemens Glaubitz .

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  1. Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS, Grenoble, France

    Valentin Gordeliy

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Kriebel, C.N., Becker-Baldus, J., Glaubitz, C. (2022). Solid-State NMR Spectroscopy on Microbial Rhodopsins. In: Gordeliy, V. (eds) Rhodopsin. Methods in Molecular Biology, vol 2501. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2329-9_9

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