Abstract
Microbial rhodopsins have become an indispensable tool for neurobiology. Of thousands of identified microbial rhodopsins, a minute fraction has been studied so far and they have shown remarkable functional diversity suggesting more great promises that this large family holds. Effective production of recombinant microbial and viral rhodopsins is a prerequisite for the success of functional and structural studies of these proteins. Escherichia coli (E. coli) are suitable for high yield expression of many of microbial and viral rhodopsins and they facilitate rapid exploration of this large protein family.
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Acknowledgments
This work was supported by Deutsche Forschungsgemeinschaft (DFG) project 430170559. V.G. greatly acknowledges the support of Hermann von Helmholtz-Gemeinschaft Deutscher Forschungszentren (HGF) within Helmholtz professorship. This work was supported by Russian Science Foundation (RSF) Project 21-64-00018.
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Balandin, T., Volkov, D., Alekseev, A., Kovalev, K., Bratanov, D., Gordeliy, V. (2022). E. coli Expression and Purification of Microbial and Viral Rhodopsins. In: Gordeliy, V. (eds) Rhodopsin. Methods in Molecular Biology, vol 2501. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2329-9_5
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