Abstract
Flowering time is one of the most important developmental transitions in plants, especially in annuals such as Arabidopsis thaliana. However, flowering is also a critical agronomic trait, as it impacts the level of vegetative biomass produced (e.g., leaves) or the amount of seed (grain) generated. Therefore, uncovering flowering phenotypes would help understand the impact of any regulatory network on the overall plant life cycle, since flowering integrates multiple cues, both environmental (e.g., photoperiod, temperature) and internal (e.g., induction/repression of specific genes, phytohormone accumulation, plant age). Although the photoperiod flowering pathway has been extensively studied, and its gene circuitry characterized in great detail, specific flowering time protocols are mostly accessible to specialized laboratories in this field. In this report, we address this knowledge gap by generating a reproducible, non-expensive, and step-by-step protocol to assess flowering time under different photoperiods. We provide a comprehensive description and highlight the major pitfalls in the process. Moreover, this protocol could be expanded to include temperature changes and thus contribute to assess the impact of both environmental conditions in the plant’s decision to flower.
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References
Koornneef M, Hanhart CJ, van der Veen JH (1991) A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana. Mol Gen Genet 229:57–66. https://doi.org/10.1007/BF00264213
Martinez-Zapater JM, Somerville CR (1990) Effect of light quality and vernalization on late-flowering mutants of Arabidopsis thaliana. Plant Physiol 92:770–776. https://doi.org/10.1104/pp.92.3.770
Mouradov A, Cremer F, Coupland G (2002) Control of flowering time: interacting pathways as a basis for diversity. Plant Cell 14(Suppl):S111–S130. https://doi.org/10.1105/tpc.001362
Wahl V, Ponnu J, Schlereth A et al (2013) Regulation of flowering by trehalose-6-phosphate signaling in Arabidopsis thaliana. Science 339:704–707. https://doi.org/10.1126/science.1230406
Xu M, Hu T, Zhao J et al (2016) Developmental functions of miR156-regulated SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes in Arabidopsis thaliana. PLoS Genet 12:e1006263. https://doi.org/10.1371/journal.pgen.1006263
McClung CR, Lou P, Hermand V, Kim JA (2016) The importance of ambient temperature to growth and the induction of flowering. Front Plant Sci 7:1266. https://doi.org/10.3389/fpls.2016.01266
Jin S, Ahn JH (2021) Regulation of flowering time by ambient temperature: repressing the repressors and activating the activators. New Phytol. https://doi.org/10.1111/nph.17217
Andrés F, Coupland G (2012) The genetic basis of flowering responses to seasonal cues. Nat Rev Genet 13:627–639. https://doi.org/10.1038/nrg3291
Davis SJ (2009) Integrating hormones into the floral-transition pathway of Arabidopsis thaliana. Plant Cell Environ 32:1201–1210. https://doi.org/10.1111/j.1365-3040.2009.01968.x
Conti L (2017) Hormonal control of the floral transition: can one catch them all? Dev Biol 430:288–301. https://doi.org/10.1016/j.ydbio.2017.03.024
Eriksson S, Böhlenius H, Moritz T, Nilsson O (2006) GA4 is the active gibberellin in the regulation of LEAFY transcription and Arabidopsis floral initiation. Plant Cell 18:2172–2181. https://doi.org/10.1105/tpc.106.042317
Plackett ARG, Powers SJ, Fernandez-Garcia N et al (2012) Analysis of the developmental roles of the Arabidopsis gibberellin 20-oxidases demonstrates that GA20ox1, -2, and -3 are the dominant paralogs. Plant Cell 24:941–960. https://doi.org/10.1105/tpc.111.095109
Osnato M, Castillejo C, Matías-Hernández L, Pelaz S (2012) TEMPRANILLO genes link photoperiod and gibberellin pathways to control flowering in Arabidopsis. Nat Commun 3:808. https://doi.org/10.1038/ncomms1810
Wang J-W (2014) Regulation of flowering time by the miR156-mediated age pathway. J Exp Bot 65:4723–4730
Whittaker C, Dean C (2017) The FLC locus: A platform for discoveries in epigenetics and adaptation. Annu Rev Cell Dev Biol 33:555–575. https://doi.org/10.1146/annurev-cellbio-100616-060546
Kardailsky I, Shukla VK, Ahn JH et al (1999) Activation tagging of the floral inducer FT. Science 286:1962–1965. https://doi.org/10.1126/science.286.5446.1962
Kobayashi Y, Weigel D (2007) Move on up, it’s time for change – mobile signals controlling photoperiod-dependent flowering. Genes Dev 21:2371–2384. https://doi.org/10.1101/gad.1589007
Song YH, Ito S, Imaizumi T (2013) Flowering time regulation: photoperiod- and temperature-sensing in leaves. Trends Plant Sci 18:575–583. https://doi.org/10.1016/j.tplants.2013.05.003
Abe M, Kobayashi Y, Yamamoto S et al (2005) FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science 309:1052–1056. https://doi.org/10.1126/science.1115983
Wigge PA, Kim MC, Jaeger KE et al (2005) Integration of spatial and temporal information during floral induction in Arabidopsis. Science 309:1056–1059. https://doi.org/10.1126/science.1114358
Bradley D, Ratcliffe O, Vincent C et al (1997) Inflorescence commitment and architecture in Arabidopsis. Science 275:80–83. https://doi.org/10.1126/science.275.5296.80
Shannon S, Meeks-Wagner DR (1991) A mutation in the Arabidopsis TFL1 gene affects inflorescence meristem development. Plant Cell 3:877–892. https://doi.org/10.1105/tpc.3.9.877
Benlloch R, Berbel A, Serrano-Mislata A, Madueño F (2007) Floral initiation and inflorescence architecture: a comparative view. Ann Bot 100:659–676. https://doi.org/10.1093/aob/mcm146
Parcy F (2005) Flowering: a time for integration. Int J Dev Biol 49:585–593. https://doi.org/10.1387/ijdb.041930fp
Fornara F, Panigrahi KCS, Gissot L et al (2009) Arabidopsis DOF transcription factors act redundantly to reduce CONSTANS expression and are essential for a photoperiodic flowering response. Dev Cell 17:75–86. https://doi.org/10.1016/j.devcel.2009.06.015
Kinoshita A, Richter R (2020) Genetic and molecular basis of floral induction in Arabidopsis thaliana. J Exp Bot 71:2490–2504. https://doi.org/10.1093/jxb/eraa057
Pouteau S, Albertini C (2009) The significance of bolting and floral transitions as indicators of reproductive phase change in Arabidopsis. J Exp Bot 60:3367–3377. https://doi.org/10.1093/jxb/erp173
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1 Electronic Supplementary Materials
Supplementary Table 1
Data scoring as leaf number (XLSX 48 kb)
Supplementary Table 2
Data scoring as days until bolting (XLSX 22 kb)
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Praena, J., van Veen, E., Henriques, R., Benlloch, R. (2022). Assessing Flowering Time Under Different Photoperiods. In: Duque, P., Szakonyi, D. (eds) Environmental Responses in Plants. Methods in Molecular Biology, vol 2494. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2297-1_7
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DOI: https://doi.org/10.1007/978-1-0716-2297-1_7
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