Abstract
Adipose tissue is highly heterogeneous and plastic. Recent advances in single-cell/nucleus RNA sequencing technology have helped to study the cellular composition and dynamics of adipose tissue. In this protocol, I outline a typical workflow of analyzing single-cell/nucleus transcriptome data. Specifically, I show an example of how cellular populations are estimated and characterized from a single-nucleus RNAseq data set of frozen archived human adipose tissue.
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Acknowledgements
I am grateful to Christian Wolfrum and Ian Theo Mitchell for editing the manuscript. This work was supported by the Swiss National Science Foundation (P2EZP3 191874).
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Sun, W. (2022). Analysis of Single-Cell/Nucleus Transcriptome Data in Adipose Tissue. In: Guertin, D.A., Wolfrum, C. (eds) Brown Adipose Tissue. Methods in Molecular Biology, vol 2448. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2087-8_19
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DOI: https://doi.org/10.1007/978-1-0716-2087-8_19
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2087-8
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