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Approaches to Monitor Termination of DNA Replication Using Xenopus Egg Extracts

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DNA Damage Responses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2444))

Abstract

DNA replication is crucial for cell viability and genome integrity. Despite its crucial role in genome duplication, the final stage of DNA replication, which is termed termination, is relatively unexplored. Our knowledge of termination is limited by cellular approaches to study DNA replication, which cannot readily detect termination. In contrast, the Xenopus laevis egg extract system allows for all of DNA replication to be readily detected. Here we describe the use of this system and assays to monitor replication termination.

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Change history

  • 11 May 2022

    The chapter was inadvertently published with the Acknowledgment section excluded in the chapter.

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Acknowledgments

JMD is supported by NIH grant R35GM128696.

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Authors and Affiliations

  1. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA

    Tamar Kavlashvili & James M. Dewar

Authors
  1. Tamar Kavlashvili
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  2. James M. Dewar
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Corresponding author

Correspondence to James M. Dewar .

Editor information

Editors and Affiliations

  1. Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA

    Nima Mosammaparast

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kavlashvili, T., Dewar, J.M. (2022). Approaches to Monitor Termination of DNA Replication Using Xenopus Egg Extracts. In: Mosammaparast, N. (eds) DNA Damage Responses. Methods in Molecular Biology, vol 2444. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2063-2_7

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  • DOI: https://doi.org/10.1007/978-1-0716-2063-2_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2062-5

  • Online ISBN: 978-1-0716-2063-2

  • eBook Packages: Springer Protocols

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