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Assessing DNA Damage Responses Using B Lymphocyte Cultures

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DNA Damage Responses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2444))

Abstract

Development of B cells requires the programmed generation and repair of double-stranded DNA breaks in antigen receptor genes. Investigation of the cellular responses to these DNA breaks has established important insights into B cell development and, more broadly, has provided fundamental advances into the molecular mechanisms of DNA damage response pathways. Abelson transformed pre-B cell lines and primary pre-B cell cultures are malleable experimental systems with diverse applications for studying DNA damage responses. This chapter describes methods for generating these cellular systems, inducing and quantifying DSBs, and assessing DNA damage programs.

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Acknowledgments

RJ is supported by a training grant through the Alvin J. Siteman Cancer Center. JJB is supported by National Institutes of Health grant R56 AI153234, an American Society of Hematology Scholar Award, the Children’s Discovery Institute at St. Louis Children’s Hospital and Washington University School of Medicine, the St. Louis Children’s Hospital Foundation, and the Gabrielle’s Angel Foundation.

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Authors and Affiliations

  1. Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA

    Rachel Johnston, Lynn S. White & Jeffrey J. Bednarski

Authors
  1. Rachel Johnston
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  2. Lynn S. White
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  3. Jeffrey J. Bednarski
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Corresponding author

Correspondence to Jeffrey J. Bednarski .

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Editors and Affiliations

  1. Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA

    Nima Mosammaparast

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Johnston, R., White, L.S., Bednarski, J.J. (2022). Assessing DNA Damage Responses Using B Lymphocyte Cultures. In: Mosammaparast, N. (eds) DNA Damage Responses. Methods in Molecular Biology, vol 2444. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2063-2_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2063-2_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2062-5

  • Online ISBN: 978-1-0716-2063-2

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