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Characterization of DNA-PK-Bound End Fragments Using GLASS-ChIP

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DNA Damage Responses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2444))

Abstract

Endonucleolytic cleavage of DNA ends by the human Mre11-Rad50-Nbs1 (MRN) complex occurs in a manner that is promoted by DNA-dependent protein kinase (DNA-PK). A method is described to isolate DNA-PK-bound fragments released from chromatin in human cells using a modified Gentle Lysis and Size Selection chromatin immunoprecipitation (GLASS-ChIP) protocol. This method, combined with real-time PCR or next-generation sequencing, can identify sites of MRN endonucleolytic cutting adjacent to DNA-PK binding sites in human cells.

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Author information

Authors and Affiliations

  1. The Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA

    Rajashree A. Deshpande & Tanya T. Paull

Authors
  1. Rajashree A. Deshpande
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  2. Tanya T. Paull
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Corresponding author

Correspondence to Tanya T. Paull .

Editor information

Editors and Affiliations

  1. Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA

    Nima Mosammaparast

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Deshpande, R.A., Paull, T.T. (2022). Characterization of DNA-PK-Bound End Fragments Using GLASS-ChIP. In: Mosammaparast, N. (eds) DNA Damage Responses. Methods in Molecular Biology, vol 2444. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2063-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2063-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2062-5

  • Online ISBN: 978-1-0716-2063-2

  • eBook Packages: Springer Protocols

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