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Antibody-Based Sensors for Pathogen Detection

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Protocols for the Diagnosis of Pig Viral Diseases

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Abstract

Antibodies are soluble biomolecules of the Immunoglobulin family found in serum, which can specifically bind to and neutralize diverse antigens. Since their discovery, antibodies have been utilized for diagnostic, therapeutic, and research purposes. The development of genetic engineering and recombinant technology has made it possible to modify antibodies in structure and composition. Antibodies have found utility in the field of diagnostics with the incorporation of native or recombinant antibodies in biosensing platforms, capable of transducing the information of an antigen-antibody binding event into a measurable signal. This platform is termed as an immunosensor. Several approaches are available for the immobilization of antibodies on the surface of the transducer. These approaches include either a covalent or non-covalent attachment of the active form of antibodies in proper orientation, while retaining the conductivity of the transducing elements at the same time. The generated signal can be an electrical, optical, shear strain, or temperature change. Accordingly, immunosensors can be broadly divided into electrochemical, optical, piezoelectric or thermometric immunosensors. Each type has its own set of advantages and challenges in the context of design and sensing efficiency.

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References

  1. Trier NH, Houen G (2020) Antibodies as diagnostic targets and as reagents for diagnostics. Antibodies (Basel) 9(2):15. https://doi.org/10.3390/antib9020015

    Article  Google Scholar 

  2. Kohler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497

    Article  CAS  Google Scholar 

  3. Lu R, Hwang Y, Liu I et al (2020) Development of therapeutic antibodies for the treatment of diseases. J Biomed Sci 27:1

    Article  CAS  Google Scholar 

  4. Kyowa Hakko Kirin Co. Ltd. Consolidated Financial Summary (IFRS) Fiscal 2018. 2019, February 5

    Google Scholar 

  5. Basu K, Green EM, Cheng Y, Craik CS (2019) Why recombinant antibodies - benefits and applications. Curr Opin Biotechnol 60:153–158. https://doi.org/10.1016/j.copbio.2019.01.012

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Gray AC, Sidhu SS, Chandrasekera PC et al (2016) Animal-friendly affinity reagents: replacing the needless in the haystack. Trends Biotechnol 34(12):960–969. https://doi.org/10.1016/j.tibtech.2016.05.017

    Article  CAS  PubMed  Google Scholar 

  7. Sharma S, Byrne H, O'Kennedy RJ (2016) Antibodies and antibody-derived analytical biosensors. Essays Biochem 60(1):9–18. https://doi.org/10.1042/EBC20150002

    Article  PubMed  PubMed Central  Google Scholar 

  8. Schroeder HW Jr, Cavacini L (2010) Structure and function of immunoglobulins. J Allergy Clin Immunol 125:S41–S52. https://doi.org/10.1016/j.jaci.2009.09.046

    Article  PubMed  PubMed Central  Google Scholar 

  9. Ma H, O’Kennedy R (2015) The structure of natural and recombinant antibodies. In: Houen G (ed) Peptide antibodies. Methods in molecular biology, vol 1348. Humana Press, New York. https://doi.org/10.1007/978-1-4939-2999-3_2

    Chapter  Google Scholar 

  10. Weiner LM, Surana R, Wang S (2010) Monoclonal antibodies: versatile platforms for cancer immunotherapy. Nat Rev Immunol 10:317–327

    Article  CAS  Google Scholar 

  11. Chiu ML, Goulet DR, Teplyakov A et al (2019) Antibody structure and function: the basis for engineering therapeutics. Antibodies 8(4):55. https://doi.org/10.3390/antib8040055

    Article  CAS  PubMed Central  Google Scholar 

  12. Reverberi R, Reverberi L (2007) Factors affecting the antigen-antibody reaction. Blood Transfus 5(4):227–240. https://doi.org/10.2450/2007.0047-07

    Article  PubMed  PubMed Central  Google Scholar 

  13. Mollarasouli F, Kurbanoglu S, Ozkan SA (2019) The role of electrochemical Immunosensors in clinical analysis. Biosensors 9(3):86. https://doi.org/10.3390/bios9030086

    Article  CAS  PubMed Central  Google Scholar 

  14. Lim SA, Ahmed MU (2019) Chapter 1: Introduction to Immunosensors, Royal Society of Chemistry. In: Ahmed MU, Zourob M, Tamiya E (eds) Immunosensors, pp 1–20. https://doi.org/10.1039/9781788016162-00001

    Chapter  Google Scholar 

  15. Clark LC Jr, Lyons C (1962) Electrode systems for continuous monitoring in cardiovascular surgery. Ann N Y Acad Sci 102:29–45. https://doi.org/10.1111/j.1749-6632.1962.tb13623.x

    Article  CAS  PubMed  Google Scholar 

  16. Yalow RS, Berson SA (1959) Assay of plasma insulin in human subjects by immunological methods. Nature 184(Suppl 21):1648–1649. https://doi.org/10.1038/1841648b0

    Article  CAS  PubMed  Google Scholar 

  17. Turner APF (2000) Biosensors—sense and sensitivity. Science 290(5495):1315–1317

    Article  CAS  Google Scholar 

  18. Skottrup PD, Nicolaisen M, Justesen AF (2008) Towards on-site pathogen detection using antibody-based sensors. Biosens Bioelectron 24(3):339–348. https://doi.org/10.1016/j.bios.2008.06.045

    Article  CAS  PubMed  Google Scholar 

  19. Singh A (2017) Next generation immunoassays for infectious diseases of animals. Res Rev J Immunol 7(2):22–43

    Google Scholar 

  20. Cass T, Ligler FS (1998) Immobilized biomolecules in analysis: A practical approach. Oxford University Press, New York

    Google Scholar 

  21. Trilling AK, Beekwilder J, Zuilhof H (2013) Antibody orientation on biosensor surfaces: a minireview. Analyst 138:1619–1627

    Article  CAS  Google Scholar 

  22. John R, Spencer M, Wallace GG et al (1991) Development of a polypyrrole-based human serum albumin sensor. Anal Chim Acta 249:381–385

    Article  CAS  Google Scholar 

  23. Feyssa B, Liedert C, Kivimaki L et al (2013) Patterned immobilization of antibodies within roll-to-roll hot embossed polymeric microfluidic channels. PLoS One 8:e68918

    Article  CAS  Google Scholar 

  24. Jarocka U, Sawicka R, Góra-Sochacka A et al (2014) An immunosensor based on antibody binding fragments attached to gold nanoparticles for the detection of peptides derived from avian influenza hemagglutinin H5. Sensors 14:15714–15728

    Article  Google Scholar 

  25. Shriver-Lake LC, Donner B, Edelstein R et al (1997) Antibody immobilization using heterobifunctional crosslinkers. Biosens Bioelectron 12:1101–1106

    Article  CAS  Google Scholar 

  26. de Juan-Franco E, Caruz A, Pedrajas JR et al (2013) Site-directed antibody immobilization using a protein A–gold binding domain fusion protein for enhanced SPR immunosensing. Analyst 138:2023–2031

    Article  Google Scholar 

  27. Barton AC, Collyer SD, Davis Fet al. (2009) Labeless AC impedimetric antibody-based sensors with pg ml−1 sensitivities for point-of-care biomedical applications. Biosens Bioelectron 24:1090–1095

    Article  CAS  Google Scholar 

  28. Ionescu RE, Gondran C, Bouffier L et al (2010) Label-free impedimetric immunosensor for sensitive detection of atrazine. Electrochim Acta 55:6228–6232

    Article  CAS  Google Scholar 

  29. Yan H, Shen Z, Mernaugh R, Zeng X (2011) Single chain fragment variable recombinant antibody as a template for fc sensors. Anal Chem 83(2):625–630. https://doi.org/10.1021/ac102087w

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Zeng X, Shen Z, Mernaugh R (2012) Recombinant antibodies and their use in biosensors. Anal Bioanal Chem 402(10):3027–3038. https://doi.org/10.1007/s00216-011-5569-z

    Article  CAS  PubMed  Google Scholar 

  31. Aydin EB, Aydin M, Sezgintürk MK (2019) Advances in electrochemical immunosensors. Adv Clin Chem 92:1–57

    Article  CAS  Google Scholar 

  32. Holford TR, Davis F, Higson SP (2012) Recent trends in antibody based sensors. Biosens Bioelectron 34(1):12–24. https://doi.org/10.1016/j.bios.2011.10.023

    Article  CAS  PubMed  Google Scholar 

  33. Chai R, Yuan R, Chai Y et al (2008) Amperometric immunosensors based on layer-by-layer assembly of gold nanoparticles and methylene blue on thiourea modified glassy carbon electrode for determination of human chorionic gonadotrophin. Talanta 74(5):1330–1336. https://doi.org/10.1016/j.talanta.2007.08.046

    Article  CAS  PubMed  Google Scholar 

  34. Fu Y, Li P, Wang T et al (2010) Novel polymeric bionanocomposites with catalytic Pt nanoparticles label immobilized for high performance amperometric immunoassay. Biosens Bioelectron 25(7):1699–1704. https://doi.org/10.1016/j.bios.2009.12.010

    Article  CAS  PubMed  Google Scholar 

  35. Bataillard P, Gardies F, Jaffrezic-Renault N et al (1988) Direct detection of immunospecies by capacitance measurements. Anal Chem 60(21):2374–2379. https://doi.org/10.1021/ac00172a011

    Article  CAS  PubMed  Google Scholar 

  36. de Moraes A, Kubota L (2016) Recent trends in field-effect transistors-based Immunosensors. Chemosensors 4(4):20. https://doi.org/10.3390/chemosensors4040020

    Article  CAS  Google Scholar 

  37. González-Martínez MA, Puchades R, Maquieira A (2007) Optical immunosensors for environmental monitoring: how far have we come? Anal Bioanal Chem 387(1):205–218. https://doi.org/10.1007/s00216-006-0849-8

    Article  CAS  PubMed  Google Scholar 

  38. Velusamy V, Arshak K, Korostynska O et al (2010) An overview of foodborne pathogen detection: in the perspective of biosensors. Biotechnol Adv 28(2):232–254. https://doi.org/10.1016/j.biotechadv.2009.12.004

    Article  CAS  PubMed  Google Scholar 

  39. Phillips KS, Cheng Q (2007) Recent advances in surface plasmon resonance based techniques for bioanalysis. Anal Bioanal Chem 387:1831–1840

    Article  CAS  Google Scholar 

  40. Bunde RL, Jarvi EJ, Rosentreter JJ (1998) Piezoelectric quartz crystal biosensors. Talanta 46(6):1223–1236. https://doi.org/10.1016/s0039-9140(97)00392-5

    Article  CAS  PubMed  Google Scholar 

  41. Pohanka M (2018) Overview of piezoelectric biosensors, Immunosensors and DNA sensors and their applications. Materials 11(3):448. https://doi.org/10.3390/ma11030448

    Article  CAS  PubMed Central  Google Scholar 

  42. Ramanathan K, Danielsson B (2001) Principles and applications of thermal biosensors. Biosens Bioelectron 16(6):417–423. https://doi.org/10.1016/s0956-5663(01)00124-5

    Article  CAS  PubMed  Google Scholar 

  43. Liu F, Choi KS, Park TJ, Lee SY, Seo TS (2011) Graphene-based electrochemical biosensor for pathogenic virus detection. BioChip J 5(2):123–128. https://doi.org/10.1007/s13206-011-5204-2

    Article  CAS  Google Scholar 

  44. Kaushik A, Yndart A, Kumar S, Jayant RD, Vashist A, Brown AN, Li CZ, Nair M (2018) A sensitive electrochemical immunosensor for label-free detection of Zika-virus protein. Sci Rep 8(9700):1–5. https://doi.org/10.1038/s41598-018-28035-3

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. School of Medical Sciences and Technology, Indian Institute of Technology, Kharagpur, West Bengal, India

    Nirmita Dutta & Gorachand Dutta

  2. Department of Veterinary Microbiology and Immunology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

    Akhil Kumar

  3. Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

    Anu Kumari, Sushila Maan & Vinay G. Joshi

Authors
  1. Nirmita Dutta
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  2. Akhil Kumar
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  3. Anu Kumari
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  4. Sushila Maan
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  5. Gorachand Dutta
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  6. Vinay G. Joshi
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Editor information

Editors and Affiliations

  1. National Research Centre on Pig, Indian Council of Agricultural Research, Guwahati, Assam, India

    Rajib Deb

  2. National Research Centre on Pig, Indian Council of Agricultural Research, Guwahati, Assam, India

    Ajay Kumar Yadav

  3. National Research Centre on Pig, Indian Council of Agricultural Research, Guwahati, Assam, India

    Swaraj Rajkhowa

  4. College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

    Yashpal Singh Malik

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Dutta, N., Kumar, A., Kumari, A., Maan, S., Dutta, G., Joshi, V.G. (2022). Antibody-Based Sensors for Pathogen Detection. In: Deb, R., Yadav, A.K., Rajkhowa, S., Malik, Y.S. (eds) Protocols for the Diagnosis of Pig Viral Diseases. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2043-4_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2043-4_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2042-7

  • Online ISBN: 978-1-0716-2043-4

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