Abstract
We describe the staining methods used for simultaneous detection of tumor microenvironment components as well as the automated quantification methodologies. This method uses mouse formalin-fixed paraffin-embedded tissues and multiplex immunofluorescence (Multiplex IF) followed by multispectral imaging. Currently, this methodology has shown to have a valuable role in murine immunoprofiling, and can be useful when evaluating the changes incurred on the tumor microenvironment upon various immunopreventive strategies.
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Acknowledgments
Work supported by grants 1R37CA237384 (PI: McAllister) and R25E (CA056452, Shine Chang) from National Cancer Institute, V Foundation for Cancer Research (Translational Award), and the Andrew Sabin Family Foundation. Additionally, part of this research was performed in the Flow Cytometry & Cellular Imaging Core Facility, which is supported in part by the National Institutes of Health through M. D. Anderson’s Cancer Center Support Grant CA016672 and the NCI’s Research Specialist 1 R50 CA243707-01A1.
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Quesada, P.R., Riquelme, E., Burks, J., Rakoski, A., Sahin, I., McAllister, F. (2022). Assessment of the Murine Tumor Microenvironment by Multiplex Immunofluorescence. In: McAllister, F. (eds) Cancer Immunoprevention. Methods in Molecular Biology, vol 2435. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2014-4_8
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DOI: https://doi.org/10.1007/978-1-0716-2014-4_8
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2013-7
Online ISBN: 978-1-0716-2014-4
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