Abstract
Axonal transport moves proteins, RNAs, and organelles between the soma and synapses to support synaptic function and activity-dependent changes in synaptic strength. This transport is impaired in several neurodegenerative disorders such as Alzheimer’s disease. Thus, it is critical to understand the regulation and underlying mechanisms of the transport process. Aplysia californica provides a powerful experimental system for studying the interplay between synaptic activity and transport because its defined synaptic circuits can be built in-vitro. Advantages include precise pre- and postsynaptic manipulation, and high-resolution imaging of axonal transport. Here, we describe methodologies for the quantitative analysis of axonal transport in Aplysia sensory neurons.
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Acknowledgments
We gratefully acknowledge funding support from NIH (5R01MH094607-05, 5R21MH108929-02), and NSF (Award number 1453799) to carry out this work.
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Badal, K., Zhao, Y., Miller, K.E., Puthanveettil, S.V. (2022). Live Imaging and Quantitative Analysis of Organelle Transport in Sensory Neurons of Aplysia Californica. In: Vagnoni, A. (eds) Axonal Transport. Methods in Molecular Biology, vol 2431. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1990-2_2
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DOI: https://doi.org/10.1007/978-1-0716-1990-2_2
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