Abstract
Immunohistochemical procedures are complex, multiparameter assays that must take into account many factors during protocol development. The overall goal of a successful immunohistochemistry protocol is to produce a specific, sensitive, robust, and reproducible stain. Each step in the protocol has an important role in this outcome. This chapter focuses on primary antibody selection and techniques for blocking various undesired staining reactions.
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Peton, L.A., Reyes-Múgica, M. (2022). Primary Antibody Selection and Blocking Techniques for Immunohistochemistry. In: Del Valle, L. (eds) Immunohistochemistry and Immunocytochemistry. Methods in Molecular Biology, vol 2422. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1948-3_6
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DOI: https://doi.org/10.1007/978-1-0716-1948-3_6
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1947-6
Online ISBN: 978-1-0716-1948-3
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