Abstract
Parasite infections caused by helminths affect hundreds of millions worldwide. Despite their socioeconomic importance and impact on health, there is still an urgent need to develop appropriate control approaches. The recent discovery that helminths, as most eukaryotic organisms, secrete extracellular vesicles (EVs) of different type has opened new avenues for the characterization of novel diagnostic and vaccine candidates that could serve for this purpose. Herein, we describe a method for the isolation of highly pure microvesicles and exosomes, two of the most relevant populations of EVs secreted by helminths, and describe a validated approach to characterize the proteins from different compartments of EVs. These proteins could be further developed into suitable diagnostic and vaccine candidates against these devastating infections.
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Acknowledgments
This work was supported by a grant from the Health Institute Carlos III (ISCIII), Ministry of Economy and Competitiveness (Spain) (MPY 406/18) and a Miguel Servet I fellowship to JS (CP17III/00002).
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Sotillo, J. (2022). MS-Based Extracellular Vesicle (EVs) Analysis: An Application to Helminth-Secreted EVs. In: Corrales, F.J., Paradela, A., Marcilla, M. (eds) Clinical Proteomics. Methods in Molecular Biology, vol 2420. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1936-0_2
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DOI: https://doi.org/10.1007/978-1-0716-1936-0_2
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