Skip to main content
SpringerLink
Log in

Microbial Identification in the Clinical Microbiology Laboratory Using MALDI-TOF-MS

  • Protocol
  • First Online:
Clinical Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2420))

Abstract

MALDI-TOF mass spectrometry has been applied with huge success to the identification of cultured microorganisms in clinical microbiology laboratories where it has become the reference method because it is simple, fast, and highly reproducible. We describe the different procedures used in the routine for pathogen identification using the Bruker MALDI Biotyper® system.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Seng P, Drancourt M, Gouriet F et al (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49:543–551. https://doi.org/10.1086/600885

    Article  CAS  PubMed  Google Scholar 

  2. Patel R (2013) Matrix-assisted laser desorption ionization-time of flight mass spectrometry in clinical microbiology. Clin Infect Dis 57:564–572. https://doi.org/10.1093/cid/cit247

    Article  CAS  PubMed  Google Scholar 

  3. Hou TY, Chiang-Ni C, Teng SH (2019) Current status of MALDI-TOF mass spectrometry in clinical microbiology. J Food Drug Anal 27:404–414

    Article  CAS  Google Scholar 

  4. Ryzhov V, Fenselau C (2001) Characterization of the protein subset desorbed by MALDI from whole bacterial cells. Anal Chem 73:746–750. https://doi.org/10.1021/ac0008791

    Article  CAS  PubMed  Google Scholar 

  5. Quiles-Melero I, García-Rodríguez J, Gómez-López A, Mingorance J (2012) Evaluation of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry for identification of Candida parapsilosis, C. orthopsilosis and C. metapsilosis. Eur J Clin Microbiol Infect Dis 31:67–71. https://doi.org/10.1007/s10096-011-1277-z

    Article  CAS  PubMed  Google Scholar 

  6. Alguacil-Guillen M, Ramos-Ruperto L, Ramos Ramos JC et al (2019) MALDI-TOF MS for rapid diagnosis of Anaerobiospirillum succiniciproducens, an unusual causative agent of bacteraemia in humans. Two case reports and literature review. Anaerobe 55:130–135. https://doi.org/10.1016/j.anaerobe.2018.12.003

    Article  CAS  PubMed  Google Scholar 

  7. Quintela Baluja M, Böhme K, Fernández-No I et al (2014) MALDI-TOF mass spectrometry, a rapid and reliable method for the identification of bacterial species in food-microbiology laboratories. Academia, pp 353–385

    Google Scholar 

  8. Clark AE, Kaleta EJ, Arora A, Wolk DM (2013) Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology. Clin Microbiol Rev 26:547–603. https://doi.org/10.1128/CMR.00072-12

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Sun Y, Guo J, Chen R et al (2021) Multicenter evaluation of three different MALDI-TOF MS systems for identification of clinically relevant filamentous fungi. Med Mycol 59:81–86. https://doi.org/10.1093/mmy/myaa037

    Article  CAS  PubMed  Google Scholar 

  10. Yi Q, Xiao M, Fan X et al (2021) Evaluation of Autof MS 1000 and Vitek MS MALDI-TOF MS system in identification of closely-related yeasts causing invasive fungal diseases. Front Cell Infect Microbiol 11:628828. https://doi.org/10.3389/fcimb.2021.628828

    Article  CAS  Google Scholar 

  11. Ma Q, Zhang Q, Yuan Y et al (2020) Evaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates. BMC Microbiol 20:318. https://doi.org/10.1186/s12866-020-02005-0

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Romero-Gomez M-P, Gomez-Gil R, Pano-Pardo JR, Mingorance J (2012) Identification and susceptibility testing of microorganism by direct inoculation from positive blood culture bottles by combining MALDI-TOF and Vitek-2 compact is rapid and effective. J Infect 65:513–520. https://doi.org/10.1016/j.jinf.2012.08.013

    Article  PubMed  Google Scholar 

  13. Oviaño M (2019) Rapid identification of microorganisms directly from positive blood cultures by MALDI-TOF MS. Enferm Infecc Microbiol Clin 37:287–289. https://doi.org/10.1016/j.eimc.2018.12.007

    Article  Google Scholar 

  14. Oviaño M, Rodríguez-Sánchez B, Gómara M et al (2018) Direct identification of clinical pathogens from liquid culture media by MALDI-TOF MS analysis. Clin Microbiol Infect 24:624–629. https://doi.org/10.1016/j.cmi.2017.09.010

    Article  CAS  PubMed  Google Scholar 

  15. Tang M, Yang J, Li Y et al (2020) Diagnostic accuracy of MALDI-TOF mass spectrometry for the direct identification of clinical pathogens from urine. Open Med 15:266–273. https://doi.org/10.1515/med-2020-0038

    Article  CAS  Google Scholar 

  16. Bishop B, Geffen Y, Plaut A et al (2018) The use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid bacterial identification in patients with smear-positive bacterial meningitis. Clin Microbiol Infect 24:171–174. https://doi.org/10.1016/j.cmi.2017.05.014

    Article  CAS  PubMed  Google Scholar 

  17. Segawa S, Sawai S, Murata S et al (2014) Direct application of MALDI-TOF mass spectrometry to cerebrospinal fluid for rapid pathogen identification in a patient with bacterial meningitis. Clin Chim Acta 435:59–61. https://doi.org/10.1016/j.cca.2014.04.024

    Article  CAS  PubMed  Google Scholar 

  18. Zboromyrska Y, Rubio E, Alejo I et al (2016) Development of a new protocol for rapid bacterial identification and susceptibility testing directly from urine samples. Clin Microbiol Infect 22:561.e1–561.e6. https://doi.org/10.1016/j.cmi.2016.01.025

    Article  CAS  Google Scholar 

  19. Ferreira L, Sanchez-Juanes F, Gonzalez-Avila M et al (2010) Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48:2110–2115. https://doi.org/10.1128/JCM.02215-09

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Simon L, Ughetto E, Gaudart A et al (2018) Direct identification of 80 percent of bacteria from blood culture bottles by matrix-assisted laser desorption ionization–time of flight mass spectrometry using a 10-minute extraction protocol. J Clin Microbiol 57:e01278-18. https://doi.org/10.1128/JCM.01278-18

    Article  Google Scholar 

  21. Alcaide F, Amlerová J, Bou G et al (2018) How to: identify non-tuberculous Mycobacterium species using MALDI-TOF mass spectrometry. Clin Microbiol Infect 24:599–603. https://doi.org/10.1016/j.cmi.2017.11.012

    Article  CAS  PubMed  Google Scholar 

  22. Wilkendorf LS, Bowles E, Buil JB et al (2020) Update on matrix-assisted laser desorption ionization–time of flight mass spectrometry identification of filamentous fungi. J Clin Microbiol 58:142. https://doi.org/10.1128/JCM.01263-20

    Article  Google Scholar 

  23. Normand AC, Becker P, Gabriel F et al (2017) Validation of a new web application for identification of fungi by use of matrix-assisted laser desorption ionization–time of flight mass spectrometry. J Clin Microbiol 55:2661–2670. https://doi.org/10.1128/JCM.00263-17

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Khot PD, Fisher MA (2013) Novel approach for differentiating shigella species and Escherichia coli by matrix-assisted laser desorption ionization–time of flight mass spectrometry. J Clin Microbiol 51:3711–3716. https://doi.org/10.1128/JCM.01526-13

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Werno AM, Christner M, Anderson TP, Murdoch DR (2012) Differentiation of Streptococcus pneumoniae from Nonpneumococcal Streptococci of the Streptococcus mitis group by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 50:2863–2867. https://doi.org/10.1128/JCM.00508-12

    Article  PubMed  PubMed Central  Google Scholar 

  26. Hong E, Bakhalek Y, Taha M-K (2019) Identification of Neisseria meningitidis by MALDI-TOF MS may not be reliable. Clin Microbiol Infect 25:717–722. https://doi.org/10.1016/j.cmi.2018.09.015

    Article  CAS  PubMed  Google Scholar 

  27. Kang L, Li N, Li P et al (2017) MALDI-TOF mass spectrometry provides high accuracy in identification of Salmonella at species level but is limited to type or subtype Salmonella serovars. Eur J Mass Spectrom 23:70–82. https://doi.org/10.1177/1469066717699216

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Servicio de Microbiología, Hospital Universitario La Paz—IdiPAZ, Madrid, Spain

    Iván Bloise, M. Teresa Corcuera, Julio García-Rodríguez & Jesús Mingorance

Authors
  1. Iván Bloise
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Teresa Corcuera
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Julio García-Rodríguez
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jesús Mingorance
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jesús Mingorance .

Editor information

Editors and Affiliations

  1. Proteomics Unit, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain

    Fernando J. Corrales

  2. Proteomics Unit, Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain

    Alberto Paradela

  3. Proteomics Unit, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain

    Miguel Marcilla

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Bloise, I., Corcuera, M.T., García-Rodríguez, J., Mingorance, J. (2022). Microbial Identification in the Clinical Microbiology Laboratory Using MALDI-TOF-MS. In: Corrales, F.J., Paradela, A., Marcilla, M. (eds) Clinical Proteomics. Methods in Molecular Biology, vol 2420. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1936-0_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-1936-0_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1935-3

  • Online ISBN: 978-1-0716-1936-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation